ArticleClinical and vaccine immunology : CVI2011
Spherical body protein 4 is a new serological antigen for global detection of Babesia bovis infection in cattle.
Article in Clinical and vaccine immunology : CVI, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Article
- Spherical Body Protein 4 fromPathogens (Basel, Switzerland) · 2023Article
- Development of a promising antigenic cocktail for the global detection of Babesia caballi in horse by ELISA.PloS one · 2023Article
- Thrombospondin-Related Anonymous Protein (TRAP) Family Expression byMicroorganisms · 2022Article
- Molecular detection of Anaplasma spp., Babesia spp. and Theileria spp. in yaks (Bos grunniens) and Tibetan sheep (Ovis aries) on the Qinghai-Tibetan Plateau, China.Parasites & vectors · 2021Article
- Serological Survey of Babesia bigemina and Babesia bovis in Cattle and Water Buffaloes from Menoufia Province, Egypt.Acta parasitologica · 2021Article
- Humoral and Cell-Mediated Immune Response Validation in Calves after a Live Attenuated Vaccine ofPathogens (Basel, Switzerland) · 2020Article
- Identification and antigenicity of the Babesia caballi spherical body protein 4 (SBP4).Parasites & vectors · 2020Article
- Characterization of a novel secretory spherical body protein in Babesia orientalis and Babesia orientalis-infected erythrocytes.Parasites & vectors · 2018Article
- A novel Babesia orientalis 135-kilodalton spherical body protein like: identification of its secretion into cytoplasm of infected erythrocytes.Parasites & vectors · 2018Article
- Spherical Body Protein 2 truncated copy 11 as a specific Babesia bovis attenuation marker.Parasites & vectors · 2018Article
- In silico and phylogenetic analyses of partial BbRAP-1, BbCP2, BbSBP-4 and BbβTUB gene sequences of Babesia bovis isolates from cattle in South Africa.BMC veterinary research · 2017Article
- Molecular and serological detection of bovine babesiosis in Indonesia.Parasites & vectors · 2017Article
- A novel modified-indirect ELISA based on spherical body protein 4 for detecting antibody during acute and long-term infections with diverse Babesia bovis strains.Parasites & vectors · 2017Article
- Article
- Expression analysis and biological characterization of Babesia sp. BQ1 (Lintan) (Babesia motasi-like) rhoptry-associated protein 1 and its potential use in serodiagnosis via ELISA.Parasites & vectors · 2016Article
- Molecular characterization of a new Babesia bovis thrombospondin-related anonymous protein (BbTRAP2).PloS one · 2013Article
- Molecular detection and identification of Babesia bovis and Babesia bigemina in cattle in northern Thailand.Parasitology research · 2012Article
- Validation and field evaluation of a competitive enzyme-linked immunosorbent assay for diagnosis of Babesia bovis infections in Argentina.Clinical and vaccine immunology : CVI · 2012Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Five Babesia bovis recombinant proteins, including merozoite surface antigen 2c (BbMSA-2c), C-terminal rhoptry-associated protein 1 (BbRAP-1/CT), truncated thrombospondin-related anonymous protein (BbTRAP-T), spherical body protein 1 (BbSBP-1), and spherical body protein 4 (BbSBP-4), were evaluated as diagnostic antigens to detect the infection in cattle. The recombinant proteins were highly antigenic when tested with experimentally B. bovis-infected bovine serum in Western blot analysis. Furthermore, five antisera that had been raised against each of the recombinant proteins reacted specifically with the corresponding authentic protein, as determined in Western blot analysis. Next, enzyme-linked immunosorbent assays (ELISAs) using these recombinant proteins were evaluated for diagnostic use, and the sensitivity and specificity of each protein were demonstrated with a series of serum samples from experimentally B. bovis-infected cattle. Furthermore, a total of 669 field serum samples collected from cattle in regions of B. bovis endemicity in seven countries were tested with the ELISAs, and the results were compared to those of an indirect fluorescent antibody test (IFAT), as a reference. Among five recombinant antigens, recombinant BbSBP-4 (rBbSBP-4) had the highest concordance rate (85.3%) and kappa value (0.705), indicating its reliability in the detection of specific antibodies to B. bovis in cattle, even in different geographical regions. Overall, we have successfully developed an ELISA based on rBbSBP-4 as a new serological antigen for a practical and sensitive test which will be applicable for epidemiologic survey and control programs in the future.
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