ArticleNucleic acids research2011
Crystal structure and centromere binding of the plasmid segregation protein ParB from pCXC100.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Structure and ion-dependent folding of k-junctions.RNA (New York, N.Y.) · 2023Article
- Chromosome segregation in Archaea: SegA- and SegB-DNA complex structures provide insights into segrosome assembly.Nucleic acids research · 2021Article
- Structure and folding of four putative kink turns identified in structured RNA species in a test of structural prediction rules.Nucleic acids research · 2021Article
- Effect of methylation of adenine NRNA biology · 2019Article
- Plasmid Localization and Partition inEcoSal Plus · 2019Review
- The role of RNA structure in translational regulation by L7Ae protein in archaea.RNA (New York, N.Y.) · 2019Article
- Fast Photochemical Oxidation of Proteins Coupled with Mass Spectrometry.Protein and peptide letters · 2019Review
- Article
- Function, expression, specificity, diversity and incompatibility of actinobacteriophage parABS systems.Molecular microbiology · 2016Article
- A critical base pair in k-turns determines the conformational class adopted, and correlates with biological function.Nucleic acids research · 2016Article
- Segrosome Complex Formation during DNA Trafficking in Bacterial Cell Division.Frontiers in molecular biosciences · 2016Review
- Specific and non-specific interactions of ParB with DNA: implications for chromosome segregation.Nucleic acids research · 2015Article
- A critical base pair in k-turns that confers folding characteristics and correlates with biological function.Nature communications · 2014Article
- Structure of a rare non-standard sequence k-turn bound by L7Ae protein.Nucleic acids research · 2014Article
- The molecular recognition of kink-turn structure by the L7Ae class of proteins.RNA (New York, N.Y.) · 2013Article
- Structure and function of AvtR, a novel transcriptional regulator from a hyperthermophilic archaeal lipothrixvirus.Journal of virology · 2013Article
- Bacterial plasmid partition machinery: a minimalist approach to survival.Current opinion in structural biology · 2012Review
- The transcription factor AmrZ utilizes multiple DNA binding modes to recognize activator and repressor sequences of Pseudomonas aeruginosa virulence genes.PLoS pathogens · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasmid pCXC100 from the Gram-positive bacterium Leifsonia xyli subsp. cynodontis uses a type Ib partition system that includes a centromere region, a Walker-type ATPase ParA and a centromere-binding protein ParB for stable segregation. However, ParB shows no detectable sequence homology to any DNA-binding motif. Here, we study the ParB centromere interaction by structural and biochemical approaches. The crystal structure of the C-terminal DNA-binding domain of ParB at 1.4 Å resolution reveals a dimeric ribbon-helix-helix (RHH) motif, supporting the prevalence of RHH motif in centromere binding. Using hydroxyl radical footprinting and quantitative binding assays, we show that the centromere core comprises nine uninterrupted 9-nt direct repeats that can be successively bound by ParB dimers in a cooperative manner. However, the interaction of ParB with a single subsite requires 18 base pairs covering one immediate repeat as well as two halves of flanking repeats. Through mutagenesis, sequence specificity was determined for each position of an 18-bp subsite. These data suggest an unique centromere recognition mechanism by which the repeat sequence is jointly specified by adjacent ParB dimers bound to an overlapped region.
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