ArticleThe Journal of biological chemistry2011
A novel mechanism of osmosensing, a salt-dependent protein-nucleic acid interaction in the cyanobacterium Synechocystis Species PCC 6803.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 30 citations in OpenAlex.
- Glucosylglycerol phosphorylase, a potential novel pathway of microbial glucosylglycerol catabolism.Applied microbiology and biotechnology · 2024Article
- Integrative analysis of the salt stress response in cyanobacteria.Biology direct · 2021Article
- Salt-TolerantFrontiers in microbiology · 2021Article
- Freshwater CyanobacteriumApplied and environmental microbiology · 2020Article
- Genomic Survey of Salt Acclimation-Related Genes in the Halophilic Cyanobacterium Euhalothece sp. Z-M001.Scientific reports · 2020Article
- Salt-Regulated Accumulation of the Compatible Solutes Sucrose and Glucosylglycerol in Cyanobacteria and Its Biotechnological Potential.Frontiers in microbiology · 2019Review
- Production of the compatible solute α-D-glucosylglycerol by metabolically engineered Corynebacterium glutamicum.Microbial cell factories · 2018Article
- Expanding the toolbox forSynthetic biology (Oxford, England) · 2018Article
- The Response Regulator Slr1588 RegulatesFrontiers in microbiology · 2017Article
- Salt acclimation of cyanobacteria and their application in biotechnology.Life (Basel, Switzerland) · 2014Review
- The SigB σ factor regulates multiple salt acclimation responses of the cyanobacterium Synechocystis sp. PCC 6803.Plant physiology · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The de novo synthesis of compatible solutes is an essential part of the cellular osmotic stress response. Upon an osmotic challenge, it is regulated by the immediate biochemical activation of preformed enzymes and by activation of gene expression. Whereas the transcriptional response has been investigated intensively, the mechanisms by which enzymes are activated in osmotic stress situations are still elusive. Here, we address this topic for the moderately halotolerant cyanobacterium Synechocystis sp. PCC 6803, which synthesizes glucosylglycerol as a compatible solute. The key enzyme of the glucosylglycerol pathway (GgpS) is inhibited by nucleic acids in a sequence- and length-independent manner. The protein binds DNA, RNA, and heparin via a salt-dependent electrostatic interaction with the negatively charged backbone of the polyanions. Mechanistically, DNA binding to the enzyme causes noncompetitive inhibition of GgpS activity. The interaction of the enzyme and nucleic acids under in vivo conditions is indicated by the co-purification of both after cross-linking in Synechocystis cells. We propose a novel mechanism of activity regulation by the nonspecific salt-dependent binding of an enzyme to nucleic acids.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.