ArticleTissue engineering. Part A2011
Autocrine-controlled formation and function of tissue-like aggregates by primary hepatocytes in micropatterned hydrogel arrays.
Article in Tissue engineering. Part A, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Bile canaliculi formation in primary hepatocytes requires α1β1 integrin-dependent adherens junction re-organization.Journal of cell science · 2025Article
- Chemotherapy treatment induces pro-invasive changes in liver ECM composition.Matrix biology : journal of the International Society for Matrix Biology · 2022Article
- Non-small cell lung carcinoma spheroid models in agarose microwells for drug response studies.Lab on a chip · 2022Article
- Engineering Hydrogels for the Development of Three-Dimensional In Vitro Models.International journal of molecular sciences · 2022Review
- Engineering Modular 3D Liver Culture MicroenvironmentsAdvanced nanobiomed research · 2022Article
- Integrative microphysiological tissue systems of cancer metastasis to the liver.Seminars in cancer biology · 2021Review
- One step fabrication of hydrogel microcapsules with hollow core for assembly and cultivation of hepatocyte spheroids.Acta biomaterialia · 2017Article
- Ductular reaction-on-a-chip: Microfluidic co-cultures to study stem cell fate selection during liver injury.Scientific reports · 2016Article
- Cell biology is different in small volumes: endogenous signals shape phenotype of primary hepatocytes cultured in microfluidic channels.Scientific reports · 2016Article
- Degradable hydrogels derived from PEG-diacrylamide for hepatic tissue engineering.Journal of biomedical materials research. Part A · 2015Article
- Covalent Modification of Synthetic Hydrogels with Bioactive Proteins via Sortase-Mediated Ligation.Biomacromolecules · 2015Article
- Using reconfigurable microfluidics to study the role of HGF in autocrine and paracrine signaling of hepatocytes.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Formation of stable small cell number three-dimensional ovarian cancer spheroids using hanging drop arrays for preclinical drug sensitivity assays.Gynecologic oncology · 2015Article
- Multi-cellular 3D human primary liver cell culture elevates metabolic activity under fluidic flow.Lab on a chip · 2015Article
- Photopatterning of hydrogel scaffolds coupled to filter materials using stereolithography for perfused 3D culture of hepatocytes.Biotechnology and bioengineering · 2015Article
- Modulation of Huh7.5 spheroid formation and functionality using modified PEG-based hydrogels of different stiffness.PloS one · 2015Article
- Capillary flow layer-by-layer: a microfluidic platform for the high-throughput assembly and screening of nanolayered film libraries.ACS nano · 2014Article
- Bioreactor technologies to support liver function in vitro.Advanced drug delivery reviews · 2014Review
- InVERT molding for scalable control of tissue microarchitecture.Nature communications · 2013Article
- A microwell cell culture platform for the aggregation of pancreatic β-cells.Tissue engineering. Part C, Methods · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The liver carries out a variety of essential functions regulated in part by autocrine signaling, including hepatocyte-produced growth factors and extracellular matrix (ECM). The local concentrations of autocrine factors are governed by a balance between receptor-mediated binding at the cell surface and diffusion into the local matrix and are thus expected to be influenced by the dimensionality of the cell culture environment. To investigate the role of growth factor and ECM-modulated autocrine signaling in maintaining appropriate primary hepatocyte survival, metabolic functions, and polarity, we created three-dimensional cultures of defined geometry using micropatterned semisynthetic polyethylene glycol-fibrinogen hydrogels to provide a mechanically compliant, nonadhesive material platform that could be modified by cell-secreted factors. We found that in the absence of exogenous peptide growth factors or ECM, hepatocytes retain the epidermal growth factor (EGF) receptor ligands (EGF and transforming growth factor-α) and the proto-oncogenic mesenchymal epithelial transition factor (c-MET) ligand hepatocyte growth factor (HGF), along with fibronectin. Further, hepatocytes cultured in this three-dimensional microenvironment maintained high levels of liver-specific functions over the 10-day culture period. Function-blocking inhibitors of α5β1 or EGF receptor dramatically reduced cell viability and function, suggesting that signaling by both these receptors is needed for in vitro survival and function of hepatocytes in the absence of other exogenous signals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.