ArticleProteins2011
In-cell protein NMR and protein leakage.
Article in Proteins, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Xanthomonas immunity proteins protect against the cis-toxic effects of their cognate T4SS effectors.EMBO reports · 2024Article
- In-Cell Dynamics: The Next Focus of All-Atom Simulations.The journal of physical chemistry. B · 2023Review
- Using NMR-detected hydrogen-deuterium exchange to quantify protein stability in cosolutes, under crowded conditionsMagnetic resonance letters · 2023Review
- Protein Fibrillation under Crowded Conditions.Biomolecules · 2022Review
- The intracellular environment affects protein-protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Insights into Protein Stability in Cell Lysate byChembiochem : a European journal of chemical biology · 2020Article
- NMR Characterization of Surface Receptor Protein Interactions in Live Cells Using Methylcellulose Hydrogels.Angewandte Chemie (International ed. in English) · 2020Article
- Controlling and quantifying protein concentration in Escherichia coli.Protein science : a publication of the Protein Society · 2019Article
- The Inescapable Effects of Ribosomes on In-Cell NMR Spectroscopy and the Implications for Regulation of Biological Activity.International journal of molecular sciences · 2019Review
- In-Cell NMR Spectroscopy of Intrinsically Disordered Proteins.Proteomics · 2019Review
- Interaction proteomics by using in-cell NMR spectroscopy.Journal of proteomics · 2019Review
- Applications of In-Cell NMR in Structural Biology and Drug Discovery.International journal of molecular sciences · 2019Review
- To be disordered or not to be disordered: is that still a question for proteins in the cell?Cellular and molecular life sciences : CMLS · 2017Review
- Selective observation of the disordered import signal of a globular protein by in-cell NMR: the example of frataxins.Protein science : a publication of the Protein Society · 2015Article
- Hydrogen exchange of disordered proteins in Escherichia coli.Protein science : a publication of the Protein Society · 2015Article
- Nano-size uni-lamellar lipodisq improved in situ auto-phosphorylation analysis of E. coli tyrosine kinase using (19)F nuclear magnetic resonance.Protein & cell · 2015Article
- Understanding the mechanism of proteasome 20S core particle gating.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Amide proton exchange of a dynamic loop in cell extracts.Protein science : a publication of the Protein Society · 2013Article
- Computer Simulations of the Bacterial Cytoplasm.Biophysical reviews · 2013Article
- Soft interactions and crowding.Biophysical reviews · 2013Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
In-cell nuclear magnetic resonance spectroscopy is a tool for studying proteins under physiologically relevant conditions. In some instances, however, protein signals from leaked protein are observed in the liquid surrounding the cells. Here, we examine the expression of four proteins in Escherichia coli. We describe the controls that should be used for in-cell NMR experiments and show that leakage is likely when the protein being studied exceeds ∼20% of the total cellular protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.