Evidence map›Paper›PMID 21120665›Full record

ArticlePflugers Archiv : European journal of physiology2011

Chemotaxis of MDCK-F cells toward fibroblast growth factor-2 depends on transient receptor potential canonical channel 1.

Anke Fabian, Thomas Fortmann, Etmar Bulk, Valerie C Bomben, Harald Sontheimer, Albrecht Schwab

Abstract read
PubMed Publisher
In one paragraph

Article in Pflugers Archiv : European journal of physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
  2. Review
  3. TRPC6 channels modulate the response of pancreatic stellate cells to hypoxia.Pflugers Archiv : European journal of physiology · 2017
    Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. Ion channels and transporters in tumour cell migration and invasion.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014
    Review
  10. Article
  11. Article
  12. Article
  13. Bradykinin promotes the chemotactic invasion of primary brain tumors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Anke FabianInstitute of Physiology II, University of Münster, Robert-Koch-Str. 27b, 48149, Münster, Germany. afabian@uni-muenster.de
Thomas Fortmann
Etmar Bulk
Valerie C Bomben
Harald Sontheimer
Albrecht Schwab
University of Münster · DEUniversity of Alabama · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Movement toward the source of a chemoattractant gradient is a basic cellular property in health and disease. Enhanced migration during metastasis involves deregulated growth factor signaling. Growth factor stimulation and cell migration converge both on the important second messenger Ca(2+). To date, the molecular identification of Ca(2+) entry pathways activated by growth factors during chemotaxis is still an open issue. We investigated the involvement of the nonselective Ca(2+) channel TRPC1 (transient receptor potential canonical 1) in FGF-2 guided chemotaxis by means of time-lapse video microscopy and by functional Ca(2+) measurements. To specifically address TRPC1 function in transformed MDCK cells we altered the expression levels by siRNA or overexpression. We report that TRPC1 channels are required for the orientation of transformed MDCK cells in FGF-2 gradients because TRPC1 knockdown or pharmacological blockade prevented chemotaxis. Stimulation with FGF-2 triggered an immediate Ca(2+) influx via TRPC1 channels that depended on phospholipase C and phosphatidylinositol 3-kinase signaling. Impeding this Ca(2+) influx abolished chemotaxis toward FGF-2. This functional connection correlated with clustering of FGF receptors and TRPC1 channels as was observed by immunolabeling. These findings show the important interplay between growth factor signaling and Ca(2+) influx in chemotaxis.

Indexed as

AndrostadienesAnimalsCalciumCalcium SignalingCell LineChemotaxisDogsEstrenesFibroblast Growth Factor 2HumansIntercellular Signaling Peptides and ProteinsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3PeptidesPyrrolidinonesReceptor, Fibroblast Growth Factor, Type 11-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dioneAndrostadienesCalciumEstrenesFibroblast Growth Factor 2Intercellular Signaling Peptides and ProteinsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3MTx4 protein, Grammostola spatulataPeptidesPyrrolidinonesReceptor, Fibroblast Growth Factor, Type 1Spider Venomstransient receptor potential cation channel, subfamily C, member 1TRPC Cation ChannelsWortmannin

Identifiers

PMID21120665
OpenAlexW2016432615

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.