ArticleRheumatology international2012
Comparative proteome analysis of peripheral blood mononuclear cells in systemic lupus erythematosus with iTRAQ quantitative proteomics.
Article in Rheumatology international, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Biomarkers of systemic lupus erythematosus identified using mass spectrometry-based proteomics: a systematic review.Journal of cellular and molecular medicine · 2017Pooled it
- Proteome signatures of inflammatory activated primary human peripheral blood mononuclear cells.Journal of proteomics · 2012Trial
- iTRAQ-based mass spectrometry screen to identify serum biomarkers in systemic lupus erythematosus.Lupus science & medicine · 2024Article
- Proteomic analyses reveal cystatin c is a promising biomarker for evaluation of systemic lupus erythematosus.Clinical proteomics · 2023Article
- Serum alpha-1-antitrypsin level in the severity prognosis of systemic lupus erythematosus patients: Systematic exploration of novel biomarker.BioMedicine · 2022Article
- A novel phosphoproteomic landscape evoked in response to type I interferon in the brain and in glial cells.Journal of neuroinflammation · 2021Article
- Comparative proteomics analysis of plasma protein in patients with neuropsychiatric systemic lupus erythematosus.Annals of translational medicine · 2020Article
- Quantitative proteomic analysis of sperm in unexplained recurrent pregnancy loss.Reproductive biology and endocrinology : RB&E · 2019Article
- Brain proteomic differences between wild-type and CD44- mice induced by chronic Toxoplasma gondii infection.Parasitology research · 2018Article
- Genomics, transcriptomics and proteomics to elucidate the pathogenesis of rheumatoid arthritis.Rheumatology international · 2017Review
- Diagnostic and prognostic tests in systemic lupus erythematosus.Best practice & research. Clinical rheumatology · 2017Review
- Integrated microRNA and protein expression analysis reveals novel microRNA regulation of targets in fetal down syndrome.Molecular medicine reports · 2016Article
- Comparative proteomic analysis of membranous nephropathy biopsy tissues using quantitative proteomics.Experimental and therapeutic medicine · 2015Article
- Determination of variation parameters as a crucial step in designing TMT-based clinical proteomics experiments.PloS one · 2015Article
- Comparative proteomic analysis of renal tissue in IgA nephropathy with iTRAQ quantitative proteomics.Biomedical reports · 2014Article
- Identification of potential microRNA-target pairs associated with osteopetrosis by deep sequencing, iTRAQ proteomics and bioinformatics.European journal of human genetics : EJHG · 2014Article
- In-depth profiling of the peripheral blood mononuclear cells proteome for clinical blood proteomics.International journal of proteomics · 2014Article
- Systemic lupus erythematosus diagnostics in the 'omics' era.International journal of clinical rheumatology · 2013Article
- The applied basic research of systemic lupus erythematosus based on the biological omics.Genes and immunity · 2013Review
- Differential proteomic analysis of renal tissue in lupus nephritis using iTRAQ reagent technology.Rheumatology international · 2012Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify and quantify protein profiles from peripheral blood mononuclear cells (PBMC) of systemic lupus erythematosus (SLE) patients with isobaric Tagging for Relative and Absolute protein Quantification (iTRAQ)-based proteomic technology and to find differentially expressed proteins in SLE. PBMC were collected from patients of six stable SLE, six active SLE, six rheumatoid arthritis (RA), and six healthy donors. After protein extraction and concentration, the pooled protein content was labeled with iTRAQ reagents and then subjected to multiple chromatographic fractionation and tandem mass spectrometry. ProteinPilot™ 3.0 software and a database of IPI (International Protein Index) human 3.62 were used for database searching and statistical analysis. A total of 452 proteins were identified. Of these, 67 unique proteins were observed twofold or more alteration in levels across groups. The proteins determined support existing knowledge and uncover novel biomarker candidates. These results indicate that iTRAQ-based technology can serve as a useful aid for identification and quantification proteins from PBMC.
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Registered trials
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