ArticleThe American journal of tropical medicine and hygiene2010
In vitro generation of Plasmodium falciparum ookinetes.
Article in The American journal of tropical medicine and hygiene, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Disruption of mitochondrial function in blood and sexual stages of Plasmodium falciparum by ferulenol.Parasites & vectors · 2025Article
- Aspartyl protease inhibition interferes with Plasmodium falciparum asexual blood-stage and early gametocyte development.Malaria journal · 2025Article
- Induction of gametocytogenesis in human malaria parasites: from stress to genome editing.Frontiers in microbiology · 2025Review
- The initiation and early development of apical-basal polarity in Toxoplasma gondii.Journal of cell science · 2024Article
- Full maturation of in vitro Plasmodium falciparum oocysts using the AlgiMatrix 3D culture system.Malaria journal · 2024Article
- The initiation and early development of the tubulin-containing cytoskeleton in the human parasiteMolecular biology of the cell · 2024Article
- Chemo-proteomics in antimalarial target identification and engagement.Medicinal research reviews · 2023Review
- The cortical microtubules of Toxoplasma gondii underlie the helicity of parasite movement.Journal of cell science · 2023Article
- Apical anchorage and stabilization of subpellicular microtubules by apical polar ring ensures Plasmodium ookinete infection in mosquito.Nature communications · 2022Article
- Structure-function analysis of cysteine residues in the plasmodium falciparum chitinase, PfCHT1.Protein science : a publication of the Protein Society · 2022Article
- Expansion microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid.PLoS biology · 2021Article
- A protein palmitoylation cascade regulates microtubule cytoskeleton integrity in Plasmodium.The EMBO journal · 2020Article
- Purification and production of Plasmodium falciparum zygotes from in vitro culture using magnetic column and Percoll density gradient.Malaria journal · 2020Article
- A Hetero-Multimeric Chitinase-ContainingFrontiers in cellular and infection microbiology · 2020Article
- Critical Steps ofFrontiers in microbiology · 2020Article
- malERA: An updated research agenda for basic science and enabling technologies in malaria elimination and eradication.PLoS medicine · 2017Review
- Bioluminescence Method forAntimicrobial agents and chemotherapy · 2017Article
- Plasmodium falciparum ookinete expression of plasmepsin VII and plasmepsin X.Malaria journal · 2016Article
- Transmission-blocking strategies: the roadmap from laboratory bench to the community.Malaria journal · 2016Review
- High-Throughput Assay and Discovery of Small Molecules that Interrupt Malaria Transmission.Cell host & microbe · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Plasmodium transmission from the human host to the mosquito depends on the ability of gametocytes to differentiate into ookinetes, the invasive form of the parasite that invades and establishes infection in the mosquito midgut. The biology of P. falciparum ookinetes is poorly understood, because sufficient quantities of this stage of this parasite species have not been obtained for detailed study. This report details methods to optimize production of P. falciparum sexual stage parasites, including ookinetes. Flow cytometric sorting was used to separate diploid/tetraploid zygotes and ookinetes from haploid gametetocytes and unfertilized gametes based on DNA content. Consistent production of 10(6)-10(7) P. falciparum ookinetes per 10 mL culture was observed, with ookinete transformation present in 10-40% of all parasite forms. Transmission electron micrographs of cultured parasites confirmed ookinete development.
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