ArticleInternational journal for parasitology2011
In vivo transfection of developmentally competent Brugia malayi infective larvae.
Article in International journal for parasitology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
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- New paradigms in research on Dirofilaria immitis.Parasites & vectors · 2023Review
- Article
- Transgenesis in parasitic helminths: a brief history and prospects for the future.Parasites & vectors · 2022Review
- CRISPR-mediated Transfection of Brugia malayi.PLoS neglected tropical diseases · 2020Article
- Recent advances in functional genomics for parasitic nematodes of mammals.The Journal of experimental biology · 2020Review
- CRISPR/Cas9 Mutagenesis and Expression of Dominant Mutant Transgenes as Functional Genomic Approaches in Parasitic Nematodes.Frontiers in genetics · 2019Review
- Development of a toolkit for piggyBac-mediated integrative transfection of the human filarial parasite Brugia malayi.PLoS neglected tropical diseases · 2018Article
- Review
- Prospects and challenges of CRISPR/Cas genome editing for the study and control of neglected vector-borne nematode diseases.The FEBS journal · 2016Review
- Rendering the Intractable More Tractable: Tools from Caenorhabditis elegans Ripe for Import into Parasitic Nematodes.Genetics · 2015Review
- Functional Characterization of a Novel Class of Morantel-Sensitive Acetylcholine Receptors in Nematodes.PLoS pathogens · 2015Article
- Functional analysis of microRNA activity in Brugia malayi.International journal for parasitology · 2015Article
- piggyBac: A vehicle for integrative DNA transformation of parasitic nematodes.Mobile genetic elements · 2013Article
- Nucleic acid transfection and transgenesis in parasitic nematodes.Parasitology · 2012Review
- Transposon-mediated chromosomal integration of transgenes in the parasitic nematode Strongyloides ratti and establishment of stable transgenic lines.PLoS pathogens · 2012Article
- A research agenda for helminth diseases of humans: basic research and enabling technologies to support control and elimination of helminthiases.PLoS neglected tropical diseases · 2012Review
- Analysis of transcriptional regulation of tetracycline responsive genes in Brugia malayi.Molecular and biochemical parasitology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Transient transfection of isolated Brugia malayi embryos by biolistics has proven to be useful in defining promoter structure and function in this parasite. However, isolated transfected embryos are developmentally incompetent. A method of producing developmentally competent transfected parasites is therefore needed. We report that L3 parasites can be chemically transfected in situ in the peritoneal cavity of a gerbil with a construct consisting of a secreted luciferase reporter gene containing a promoter, the 3' untranslated region and first intron derived from the B. malayi 70 kDa heat shock protein gene. The in situ chemically transfected parasites are developmentally competent, producing adult parasites with an efficiency similar to that obtained from implanted untreated L3s. Cultured adult parasites and progeny microfilariae (mf) derived from L3s transfected with this construct secreted luciferase into the culture medium. When the transfected mf were fed to mosquitoes and the resulting L3s collected, the L3s also secreted luciferase into the culture medium. Progeny mf from transgenic adult parasites contained transgenic DNA, and the transgenic mRNA produced in these parasites was found to be correctly cis- and trans-spliced. In situ chemical transformation thus results in developmentally competent transfected B. malayi in which the transgenic sequences remain transcriptionally active in all life cycle stages and are present in the subsequent generation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.