ArticleBMC genetics2010
High resolution mapping and positional cloning of ENU-induced mutations in the Rw region of mouse chromosome 5.
Article in BMC genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
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- The midbody component Prc1-like is required for microtubule reorganization during cytokinesis and dorsal determinant segregation in the early zebrafish embryo.Development (Cambridge, England) · 2023Article
- Aberrant Gene Expression Profiling in Men With Sertoli Cell-Only Syndrome.Frontiers in immunology · 2022Article
- Integrative bioinformatics approaches for identifying potential biomarkers and pathways involved in non-obstructive azoospermia.Translational andrology and urology · 2021Article
- Zebrafish as a model for kidney function and disease.Pediatric nephrology (Berlin, Germany) · 2019Review
- High Expression of Human AugminComplex Submit 3 Indicates Poor Prognosis and Associates with Tumor Progression in Hepatocellular Carcinoma.Journal of Cancer · 2019Article
- Targeting nuclear thymidylate biosynthesis.Molecular aspects of medicine · 2017Review
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- TBC1D14 regulates autophagy via the TRAPP complex and ATG9 traffic.The EMBO journal · 2016Article
- Conserved overlapping gene arrangement, restricted expression, and biochemical activities of DNA polymerase ν (POLN).The Journal of biological chemistry · 2015Article
- Genetic Defects in TAPT1 Disrupt Ciliogenesis and Cause a Complex Lethal Osteochondrodysplasia.American journal of human genetics · 2015Article
- Article
- Mouse large-scale phenotyping initiatives: overview of the European Mouse Disease Clinic (EUMODIC) and of the Wellcome Trust Sanger Institute Mouse Genetics Project.Mammalian genome : official journal of the International Mammalian Genome Society · 2012Review
- Protein degradation and iron homeostasis.Biochimica et biophysica acta · 2012Review
- ENU mutagenesis in mice identifies candidate genes for hypogonadism.Mammalian genome : official journal of the International Mammalian Genome Society · 2012Article
- Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA.The Journal of biological chemistry · 2011Article
- An ENU-mutagenesis screen in the mouse: identification of novel developmental gene functions.PloS one · 2011Article
- Mthfs is an Essential Gene in Mice and a Component of the Purinosome.Frontiers in genetics · 2011Article
Corrections and comments
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Authors and funding
24 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundForward genetic screens in mice provide an unbiased means to identify genes and other functional genetic elements in the genome. Previously, a large scale ENU mutagenesis screen was conducted to query the functional content of a ~50 Mb region of the mouse genome on proximal Chr 5. The majority of phenotypic mutants recovered were embryonic lethals.
resultsWe report the high resolution genetic mapping, complementation analyses, and positional cloning of mutations in the target region. The collection of identified alleles include several with known or presumed functions for which no mutant models have been reported (Tbc1d14, Nol14, Tyms, Cad, Fbxl5, Haus3), and mutations in genes we or others previously reported (Tapt1, Rest, Ugdh, Paxip1, Hmx1, Otoe, Nsun7). We also confirmed the causative nature of a homeotic mutation with a targeted allele, mapped a lethal mutation to a large gene desert, and localized a spermiogenesis mutation to a region in which no annotated genes have coding mutations. The mutation in Tbc1d14 provides the first implication of a critical developmental role for RAB-GAP-mediated protein transport in early embryogenesis.
conclusionThis collection of alleles contributes to the goal of assigning biological functions to all known genes, as well as identifying novel functional elements that would be missed by reverse genetic approaches.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.