ArticleBMC developmental biology2010
Hedgehog signaling is required at multiple stages of zebrafish tooth development.
Article in BMC developmental biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- scpp5 regulates tooth development and injury-induced repair in zebrafish through mineralization and Wnt/β-catenin signaling.Molecules and cells · 2026Article
- In vitro reconstitution of vertebrate Sonic Hedgehog protein cholesterolysis.bioRxiv : the preprint server for biology · 2026Article
- Zebrafish Models for Skeletal and Extraskeletal Osteogenesis Imperfecta Features: Unveiling Pathophysiology and Paving the Way for Drug Discovery.Calcified tissue international · 2024Review
- The conundrum of pharyngeal teeth origin: the role of germ layers, pouches, and gill slits.Biological reviews of the Cambridge Philosophical Society · 2022Review
- Mechanistic Insights into Axenfeld-Rieger Syndrome from ZebrafishInternational journal of molecular sciences · 2021Review
- Review
- Multiple epithelia are required to develop teeth deep inside the pharynx.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Sonic Hedgehog Signaling and Tooth Development.International journal of molecular sciences · 2020Review
- Oral and Palatal Dentition of Axolotl Arises From a Common Tooth-Competent Zone Along the Ecto-Endodermal Boundary.Frontiers in cell and developmental biology · 2020Article
- Sonic Hedgehog Regulation of the Neural Precursor Cell Fate During Chicken Optic Tectum Development.Journal of molecular neuroscience : MN · 2018Article
- Early development and replacement of the stickleback dentition.Journal of morphology · 2016Article
- Tooth and scale morphogenesis in shark: an alternative process to the mammalian enamel knot system.BMC evolutionary biology · 2015Article
- Making teeth to order: conserved genes reveal an ancient molecular pattern in paddlefish (Actinopterygii).Proceedings. Biological sciences · 2015Article
- Hedgehog signaling regulates dental papilla formation and tooth size during zebrafish odontogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2015Article
- Enhanced dentin-like mineralized tissue formation by AdShh-transfected human dental pulp cells and porous calcium phosphate cement.PloS one · 2013Article
- Retinoic acid expands the evolutionarily reduced dentition of zebrafish.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- The homology of odontodes in gnathostomes: insights from Dlx gene expression in the dogfish, Scyliorhinus canicula.BMC evolutionary biology · 2011Article
- From molecules to mastication: the development and evolution of teeth.Wiley interdisciplinary reviews. Developmental biologyReview
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe accessibility of the developing zebrafish pharyngeal dentition makes it an advantageous system in which to study many aspects of tooth development from early initiation to late morphogenesis. In mammals, hedgehog signaling is known to be essential for multiple stages of odontogenesis; however, potential roles for the pathway during initiation of tooth development or in later morphogenesis are incompletely understood.
resultsWe have identified mRNA expression of the hedgehog ligands shha and the receptors ptc1 and ptc2 during zebrafish pharyngeal tooth development. We looked for, but did not detect, tooth germ expression of the other known zebrafish hedgehog ligands shhb, dhh, ihha, or ihhb, suggesting that as in mammals, only Shh participates in zebrafish tooth development. Supporting this idea, we found that morphological and gene expression evidence of tooth initiation is eliminated in shha mutant embryos, and that morpholino antisense oligonucleotide knockdown of shha, but not shhb, function prevents mature tooth formation. Hedgehog pathway inhibition with the antagonist compound cyclopamine affected tooth formation at each stage in which we applied it: arresting development at early stages and disrupting mature tooth morphology when applied later. These results suggest that hedgehog signaling is required continuously during odontogenesis. In contrast, over-expression of shha had no effect on the developing dentition, possibly because shha is normally extensively expressed in the zebrafish pharyngeal region.
conclusionWe have identified previously unknown requirements for hedgehog signaling for early tooth initiation and later morphogenesis. The similarity of our results with data from mouse and other vertebrates suggests that despite gene duplication and changes in the location of where teeth form, the roles of hedgehog signaling in tooth development have been largely conserved during evolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.