ArticleMolecular plant pathology2011
Identification of virulence genes in the corn pathogen Colletotrichum graminicola by Agrobacterium tumefaciens-mediated transformation.
Article in Molecular plant pathology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Confrontations of the Pathogenic Fungus Colletotrichum graminicola With a Biocontrol Bacterium or a Ubiquitous Fungus Trigger Synthesis of Secondary Metabolites With Lead Structures of Synthetic Fungicides.Environmental microbiology · 2025Article
- Natural Urease Inhibitors Reduce the Severity of Disease Symptoms, Dependent on the Lifestyle of the Pathogens.Journal of fungi (Basel, Switzerland) · 2023Article
- Identifying pathogenicity-related genes in the pathogenFrontiers in microbiology · 2023Article
- Aspartate Transaminase AST2 Involved in Sporulation and Necrotrophic Pathogenesis in the HemibiotrophsFrontiers in microbiology · 2022Article
- Clathrin Is Important for Virulence Factors Delivery in the Necrotrophic FungusFrontiers in plant science · 2021Article
- Involvement of the Mitochondrial Protein Tyrosine Phosphatase PTPM1 in the Promotion of Conidiation, Development, and Pathogenicity inFrontiers in microbiology · 2020Article
- A Similar Secretome Disturbance as a Hallmark of Non-pathogenicFrontiers in microbiology · 2019Article
- Rhamnose synthase activity is required for pathogenicity of the vascular wilt fungus Verticillium dahliae.Molecular plant pathology · 2017Article
- Identification of factors involved in dimorphism and pathogenicity of Zymoseptoria tritici.PloS one · 2017Article
- Identification of Conidiogenesis-Associated Genes in Colletotrichum gloeosporioides by Agrobacterium tumefaciens-Mediated Transformation.Current microbiology · 2016Article
- Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety.BMC microbiology · 2016Article
- The Transient Receptor Potential (TRP) Channel Family in Colletotrichum graminicola: A Molecular and Physiological Analysis.PloS one · 2016Article
- Use of Random T-DNA Mutagenesis in Identification of GeneFrontiers in microbiology · 2016Article
- Genetic transformation of the tomato pathogen Pyrenochaeta lycopersici allowed gene knockout using a split-marker approach.Current genetics · 2015Article
- Article
- Agrobacterium tumefaciens-mediated transformation of the causative agent of Valsa canker of apple tree Valsa mali var. mali.Current microbiology · 2014Article
- Draft Genome Sequence of Umbilicaria muehlenbergii KoLRILF000956, a Lichen-Forming Fungus Amenable to Genetic Manipulation.Genome announcements · 2014Article
- The pectate lyase encoded by the pecCl1 gene is an important determinant for the aggressiveness of Colletotrichum lindemuthianum.Journal of microbiology (Seoul, Korea) · 2013Article
- Two recently duplicated maize NAC transcription factor paralogs are induced in response to Colletotrichum graminicola infection.BMC plant biology · 2013Article
- Agrobacterium tumefaciens-mediated transformation of the lichen fungus, Umbilicaria muehlenbergii.PloS one · 2013Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A previously developed Agrobacterium tumefaciens-mediated transformation (ATMT) protocol for the plant pathogenic fungus Colletotrichum graminicola led to high rates of tandem integration of the whole Ti-plasmid, and was therefore considered to be unsuitable for the identification of pathogenicity and virulence genes by insertional mutagenesis in this pathogen. We used a modified ATMT protocol with acetosyringone present only during the co-cultivation of C. graminicola and A. tumefaciens. Analysis of 105 single-spore isolates randomly chosen from a collection of approximately 2000 transformants, indicated that almost 70% of the transformants had single T-DNA integrations. Of 500 independent transformants tested, 10 exhibited attenuated virulence in infection assays on whole plants. Microscopic analyses primarily revealed defects at different pre-penetration stages of infection-related morphogenesis. Three transformants were characterized in detail. The identification of the T-DNA integration sites was performed by amplification of genomic DNA ends after endonuclease digestion and polynucleotide tailing. In one transformant, the T-DNA had integrated into the 5'-flank of a gene with similarity to allantoicase genes of other Ascomycota. In the second and third transformants, the T-DNA had integrated into an open reading frame (ORF) and into the 5'-flank of an ORF. In both cases, the ORFs have unknown function.
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