Evidence map›Paper›PMID 21118219›Full record

ArticleJournal of microscopy2011

A semiautomated approach for artefact removal in serial tissue cryosections.

L M Kindle, I A Kakadiaris, T Ju, J P Carson

Abstract read
In one paragraph

Article in Journal of microscopy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. A chicken embryo cardiac outflow tract atlas for registering changes due to abnormal blood flow.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2016
    Article
  7. Article
  8. Lossless 3-D reconstruction and registration of semi-quantitative gene expression data in the mouse brain.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2011
    Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

L M KindleBiological Monitoring and Modeling Group, Pacific Northwest National Laboratory, Richland, Washington, USA.
I A Kakadiaris
T Ju
J P Carson

Funding

An Interactive Volumetric Atlas of the Mouse BrainR21NS058553 · NINDS · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI CARSON, JAMES PAUL · 2009 to 2010
$391k
NINDS NIH HHS 1R21NS058553-01NINDS NIH HHS R21 NS058553
6 · The paper itself

Abstract

Thinly sliced serial tissue sections of an organ can be imaged using optical microscopy at a resolution detailing individual cells. When the tissue sections are first subjected to in situ hybridization or immunohistochemistry, these data sets can be analysed for changes in gene expression and gene products. Such spatial information is important for understanding the functional effects of experimental or environmental challenges to the organism. However, a critical step in analysing these data sets is mitigating artefacts that result from the preparation of the tissue sections. In this paper, we describe an automated method with manual validation tools that together enable detecting and addressing artefacts including dust particles and air bubbles.

Indexed as

ArtifactsAutomation, LaboratoryCryoultramicrotomyHistocytochemistryImmunohistochemistryMicroscopy

Identifiers

PMID21118219
PMCPMC3297619

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.