Evidence map›Paper›PMID 21117663›Full record

ArticleLangmuir : the ACS journal of surfaces and colloids2011

Isoelectric focusing in a drop.

Noah G Weiss, Mark A Hayes, Antonio A Garcia, Rafat R Ansari

Abstract read
In one paragraph

Article in Langmuir : the ACS journal of surfaces and colloids, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Noah G WeissDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-9709, United States.
Mark A Hayes
Antonio A Garcia
Rafat R Ansari
Arizona State University · USGlenn Research Center · US

Funding

MODULAR SURFACE PLASMON RESONANCE BIOSENSOR FOR IN-VITRO DETERMINATION OF PROTEINR01EB004761 · NIBIB · UNIVERSITY OF DELAWARE · PI BOOKSH, KARL S. · 2004 to 2010
$1.9M
PLATFORM FOR ISOLATION AND CONCENTRATION OF BIOMARKERSR21EB010191 · NIBIB · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI HAYES, MARK A · 2010 to 2011
$407k
Pathogen Isolation and Concentration for Phenotypic SubtypingR03AI099740 · NIAID · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI HAYES, MARK A · 2012 to 2013
$138k
NIAID NIH HHS R03 AI099740NIBIB NIH HHS 2R01EB004761-06NIBIB NIH HHS R01 EB004761NIBIB NIH HHS R21 EB010191NIBIB NIH HHS R21EB010191-01A1
6 · The paper itself

Abstract

A novel approach to molecular separations is investigated using a technique termed droplet-based isoelectric focusing. Drops are manipulated discretely on a superhydrophobic surface, subjected to low voltages for isoelectric focusing, and split-resulting in a preparative separation. A universal indicator dye demonstrates the generation of stable, reversible pH gradients (3-10) in ampholyte buffers, and these gradients lead to protein focusing within the drop length. Focusing was visually characterized, spectroscopically verified, and assessed quantitatively by noninvasive light scattering measurements. It was found to correlate with a quantitative model based on 1D steady-state theory. This work illustrates that molecular separations can be deployed within a single open drop, and the differential fractions can be separated into new discrete liquid elements.

Indexed as

Hydrogen-Ion ConcentrationIsoelectric FocusingLightMicrofluidic Analytical TechniquesMyoglobinScattering, RadiationMyoglobin

Identifiers

PMID21117663
PMCPMC3025497
OpenAlexW1985555497

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.