ArticleMolecular biology reports2011
Comparison of reverse transcription loop-mediated isothermal amplification, conventional PCR and real-time PCR assays for Japanese encephalitis virus.
Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 34 citations in OpenAlex.
- Arthropod-Borne Flaviviruses in Pregnancy.Microorganisms · 2023Review
- Development of Loop-Mediated Isothermal Amplification Assay TargetingFrontiers in microbiology · 2021Article
- Validation of a Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Invasive Pneumococcal Disease.Frontiers in cellular and infection microbiology · 2020Article
- Rapid, Sensitive Detection of Bartonella quintana by Loop-Mediated Isothermal Amplification of the groEL Gene.International journal of molecular sciences · 2016Article
- Article
- Rapid and sensitive detection of Listeria ivanovii by loop-mediated isothermal amplification of the smcL gene.PloS one · 2014Article
- Expression of enterovirus 71 capsid protein VP1 in Escherichia coli and its clinical application.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2013Article
- Development and application of a simple loop-mediated isothermal amplification method on rapid detection of Listeria monocytogenes strains.Molecular biology reports · 2012Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed and evaluated a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting Japanese encephalitis virus (JEV). The sensitivity of the JEV RT-LAMP assay was in concordance with that of real-time RT-PCR and 10-fold more sensitive than that of conventional RT-PCR, which the detection limit was 24 copies/μl. The JEV RT-LAMP was highly specific, which no cross-reactivity was found with dengue-2 virus, rabies virus, norovirus, astrovirus and human enterovirus 71. The JEV RT-LAMP assay was more simple and less time-consuming compared to the conventional RT-PCR and real-time RT-PCR, which the amplification could be completed in a single tube within 1 h under isothermal conditions at temperature of 63°C. The results suggest that the RT-LAMP assay can be applied as a practical molecular diagnostic tool for JEV infection and surveillance.
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