ArticleJournal of lipid research2011
A novel ELISA for measuring CD36 protein in human adipose tissue.
Article in Journal of lipid research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Acute Female Hypogonadism Alters Adipose Tissue Fatty Acid Storage Factors and Chylomicronemia.The Journal of clinical endocrinology and metabolism · 2016Trial
- Adipose Tissue Free Fatty Acid Storage In Vivo: Effects of Insulin Versus Niacin as a Control for Suppression of Lipolysis.Diabetes · 2015Trial
- Effects of male hypogonadism on regional adipose tissue fatty acid storage and lipogenic proteins.PloS one · 2012Trial
- A Multi-Omics Analysis of Metastatic Melanoma Identifies a Germinal Center-Like Tumor Microenvironment in HLA-DR-Positive Tumor Areas.Frontiers in oncology · 2021Article
- Zinc transporter Slc39a14 regulates inflammatory signaling associated with hypertrophic adiposity.American journal of physiology. Endocrinology and metabolism · 2016Article
- Adipose tissue fatty acid storage factors: effects of depot, sex and fat cell size.International journal of obesity (2005) · 2015Article
- Sex and depot differences in ex vivo adipose tissue fatty acid storage and glycerol-3-phosphate acyltransferase activity.American journal of physiology. Endocrinology and metabolism · 2015Article
- Effects of increased free fatty acid availability on adipose tissue fatty acid storage in men.The Journal of clinical endocrinology and metabolism · 2014Article
- Free fatty acid uptake in humans with CD36 deficiency.Diabetes · 2014Article
- Adipocyte mitochondrial function is reduced in human obesity independent of fat cell size.The Journal of clinical endocrinology and metabolism · 2014Article
- Adipocyte fatty acid storage factors enhance subcutaneous fat storage in postmenopausal women.Diabetes · 2013Article
- Storage rates of circulating free fatty acid into adipose tissue during eating or walking in humans.Diabetes · 2012Article
- Free fatty acid storage in human visceral and subcutaneous adipose tissue: role of adipocyte proteins.Diabetes · 2011Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
CD36 is a transmembrane protein present in many tissues that is believed to facilitate inward fatty acid transport. Western blotting is the most widely used method to measure tissue CD36 protein content, but it is time consuming, technically demanding, and semiquantitative. To more precisely measure adipose tissue CD36 content we developed an enzyme linked immunosorbent assay (ELISA) after establishing that: 1) the anti-CD36 antibodies gave a single distinct band on traditional Western blots, and 2) the vast majority of adipocyte CD36 resides in the plasma membrane. By using serial dilutions of each sample and including a calibrator sample and quality control sample on each plate, we could achieve inter- and intra-assay variability of ∼ 10%. We found that CD36 content in omental and abdominal subcutaneous adipose tissue varied over a 2-5-fold range depending upon the means of data expression (per units of tissue protein, weight, or lipid). Omental CD36 content in women decreased markedly (P = 0.01) as a function of fat cell size. For the most part, tissue CD36 content was not correlated with CD36 mRNA. This ELISA method for tissue CD36 content should enhance research into the role of this protein on tissue fatty acid uptake.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.