ArticleProceedings of the National Academy of Sciences of the United States of America2010
In vivo imaging of hydrogen peroxide production in a murine tumor model with a chemoselective bioluminescent reporter.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 136 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
136 citing papers in PubMed, 396 citations in OpenAlex.
- Beyond DCFH-DA: A Critical Review of Hydrogen Peroxide and Superoxide Detection Strategies in Mammalian Living Systems (2015-2026).International journal of molecular sciences · 2026Review
- A non-enzymatic sensor based on rGO/Pt NPs/Fc-Tyr/POPD nanocomposite for hydrogen peroxide determination in liver cancer tissues.Mikrochimica acta · 2026Article
- A Computational Modeling of ADLumin Chemiluminescence: Oxygenation and Dioxetanone Formation.Journal of computational chemistry · 2026Article
- The Pro-Metastatic Roles of ROS.Antioxidants (Basel, Switzerland) · 2026Review
- Exploiting the Clinical Potential of a Chemiluminescent Probe forChemical & biomedical imaging · 2026Article
- A guide to reactive oxygen species in tumour hypoxia: measurement and therapeutic implications.Molecular oncology · 2025Review
- Detection and Analysis of Reactive Oxygen Species (ROS): Buffer Components Are Not Bystanders.Analytical chemistry · 2025Article
- Reactive Cysteines in Proteins are the Dominant Reductants for Platinum(IV) Prodrug Activation in Live Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Intratumour oxidative hotspots provide a niche for cancer cell dissemination.Nature cell biology · 2025Article
- Article
- Hydrophilic Ethylene Glycol Fragments: A Determinant Affecting the Therapeutic Index of Paclitaxel Prodrug Nanoassemblies.ACS central science · 2024Article
- Small molecule probes for peroxynitrite detection.Redox biochemistry and chemistry · 2024Article
- Yes-associated protein-mediated melatonin regulates the function of periodontal ligament stem cells under oxidative stress conditions.World journal of stem cells · 2024Article
- The oxygen puzzle in FLASH radiotherapy: A comprehensive review and experimental outlook.Clinical and translational radiation oncology · 2024Review
- Oxidative Stress and Age-Related Tumors.Antioxidants (Basel, Switzerland) · 2024Review
- Development of a Novel Amplifiable System to Quantify Hydrogen Peroxide in Living Cells.Journal of the American Chemical Society · 2024Article
- Bioluminescence Imaging of Potassium Ion Using a Sensory Luciferin and an Engineered Luciferase.Journal of the American Chemical Society · 2024Article
- Health position paper and redox perspectives - Disease burden by transportation noise.Redox biology · 2024Review
- Current advances in the development of bioluminescent probes toward spatiotemporal trans-scale imaging.Biophysics and physicobiology · 2024Review
- In vivo bioluminescence imaging of labile iron in xenograft models and liver using FeAL-1, an iron-activatable form of D-luciferin.Cell chemical biology · 2023Article
76 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Living organisms produce hydrogen peroxide (H(2)O(2)) to kill invading pathogens and for cellular signaling, but aberrant generation of this reactive oxygen species is a hallmark of oxidative stress and inflammation in aging, injury, and disease. The effects of H(2)O(2) on the overall health of living animals remain elusive, in part owing to a dearth of methods for studying this transient small molecule in vivo. Here we report the design, synthesis, and in vivo applications of Peroxy Caged Luciferin-1 (PCL-1), a chemoselective bioluminescent probe for the real-time detection of H(2)O(2) within living animals. PCL-1 is a boronic acid-caged firefly luciferin molecule that selectively reacts with H(2)O(2) to release firefly luciferin, which triggers a bioluminescent response in the presence of firefly luciferase. The high sensitivity and selectivity of PCL-1 for H(2)O(2), combined with the favorable properties of bioluminescence for in vivo imaging, afford a unique technology for real-time detection of basal levels of H(2)O(2) generated in healthy, living mice. Moreover, we demonstrate the efficacy of PCL-1 for monitoring physiological fluctuations in H(2)O(2) levels by directly imaging elevations in H(2)O(2) within testosterone-stimulated tumor xenografts in vivo. The ability to chemoselectively monitor H(2)O(2) fluxes in real time in living animals offers opportunities to dissect H(2)O(2)'s disparate contributions to health, aging, and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.