ArticleApplied and environmental microbiology2011
Molecular description and industrial potential of Tn6098 conjugative transfer conferring alpha-galactoside metabolism in Lactococcus lactis.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 32 citations in OpenAlex.
- The lactococcal ICE-ome encodes a repertoire of exchangeable traits with potential industrial relevance.BMC genomics · 2024Article
- Detection and classification of the integrative conjugative elements of Lactococcus lactis.BMC genomics · 2024Review
- Effect of Lactic Acid Bacteria on the Lipid Profile of Bean-Based Plant Substitute of Fermented Milk.Microorganisms · 2020Article
- Visualisation of dCas9 target search in vivo using an open-microscopy framework.Nature communications · 2019Article
- Characterization of a relaxase belonging to the MOBMobile DNA · 2019Article
- TheFrontiers in microbiology · 2019Article
- Versatile Cas9-Driven Subpopulation Selection Toolbox for Lactococcus lactis.Applied and environmental microbiology · 2018Article
- The Lactococcus lactis KF147 nonribosomal peptide synthetase/polyketide synthase system confers resistance to oxidative stress during growth on plant leaf tissue lysate.MicrobiologyOpen · 2018Article
- Disruption of a Transcriptional Repressor by an Insertion Sequence Element Integration Leads to Activation of a Novel Silent Cellobiose Transporter in Lactococcus lactis MG1363.Applied and environmental microbiology · 2017Article
- From Genome to Phenotype: An Integrative Approach to Evaluate the Biodiversity of Lactococcus lactis.Microorganisms · 2017Review
- Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Microbial bioinformatics for food safety and production.Briefings in bioinformatics · 2016Review
- The carbohydrate metabolism signature of lactococcus lactis strain A12 reveals its sourdough ecosystem origin.Applied and environmental microbiology · 2013Article
- Microbial domestication signatures of Lactococcus lactis can be reproduced by experimental evolution.Genome research · 2012Article
- Systems solutions by lactic acid bacteria: from paradigms to practice.Microbial cell factories · 2011Review
- Genomic diversity and versatility of Lactobacillus plantarum, a natural metabolic engineer.Microbial cell factories · 2011Review
- Genome-scale diversity and niche adaptation analysis of Lactococcus lactis by comparative genome hybridization using multi-strain arrays.Microbial biotechnology · 2011Article
- Lactic acid bacteria: life after genomics.Microbial biotechnology · 2011Article
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4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel 51-kb conjugative transposon of Lactococcus lactis, designated Tn6098, encoding the capacity to utilize α-galactosides such as raffinose and stachyose, was identified and characterized. Alpha-galactosides are a dominant carbon source in many plant-derived foods. Most dairy lactococcus strains are unable to use α-galactosides as a growth substrate, yet many of these strains are known to have beneficial industrial traits. Conjugal transfer of Tn6098 was demonstrated from the plant-derived donor strain L. lactis KF147 to the recipient L. lactis NZ4501, a derivative of the dairy model strain L. lactis MG1363. The integration of Tn6098 into the genome of the recipient strain was confirmed by Illumina sequencing of the transconjugant L. lactis NIZO3921. The molecular structure of the integration site was confirmed by a PCR product spanning the insertion site. A 15-bp direct repeat sequence (TTATACCATAATTAC) is present on either side of Tn6098 in the chromosome of L. lactis KF147. One copy of this sequence is also present in the L. lactis MG1363 chromosome and represents the sole integration site. Phenotypic characterization of all strains showed that the transconjugant has not only acquired the ability to grow well in soy milk, a substrate rich in α-galactosides, but also has retained the flavor-forming capabilities of the recipient strain L. lactis MG1363. This study demonstrates how (induced) conjugation can be used to exploit the beneficial industrial traits of industrial dairy lactic acid bacteria in fermentation of plant-derived substrates.
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