ArticleJournal of bacteriology2011
Citrate uptake in exchange with intermediates in the citrate metabolic pathway in Lactococcus lactis IL1403.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Validation and Application of Quantitative Methods for D-Lactic Acid and L-Lactic Acid Determination in Lactic Acid Bacteria.Foods (Basel, Switzerland) · 2026Article
- Spontaneous fermentation of Tunisian dromedary milk: VASE™-GC-MS volatile profiling, physicochemical and mineral composition, bioactivities, and lactic acid bacteria enumeration.Frontiers in nutrition · 2026Article
- Recycling of Undigested Proteins Provided by the Host to the Large Intestine Microbiota: Implication for Intestinal Bacterial Anabolism, Growth, and Physiology.Microorganisms · 2025Review
- Synthetic microbial consortium enhances acetoin production and functional quality of citrus vinegar via metabolic and process optimization.Frontiers in microbiology · 2025Article
- Citrate metabolism in lactic acid bacteria: is there a beneficial effect forFrontiers in microbiology · 2023Review
- Functional Verification of the Citrate Transporter Gene in a Wine Lactic Acid Bacterium,Frontiers in bioengineering and biotechnology · 2022Article
- Identification of Potential Citrate Metabolism Pathways inMicroorganisms · 2021Article
- Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications.FEMS microbiology letters · 2020Review
- Article
- Production of buttery-odor compounds and transcriptome response in Leuconostoc gelidum subsp. gasicomitatum LMG18811T during growth on various carbon sources.Applied and environmental microbiology · 2015Article
- Ca2+-citrate uptake and metabolism in Lactobacillus casei ATCC 334.Applied and environmental microbiology · 2013Article
- Uptake of α-ketoglutarate by citrate transporter CitP drives transamination in Lactococcus lactis.Applied and environmental microbiology · 2013Article
- Rerouting citrate metabolism in Lactococcus lactis to citrate-driven transamination.Applied and environmental microbiology · 2012Article
- Substrate specificity of the citrate transporter CitP of Lactococcus lactis.Journal of bacteriology · 2012Article
- Fine tuning of the lactate and diacetyl production through promoter engineering in Lactococcus lactis.PloS one · 2012Article
- Mechanism of citrate metabolism by an oxaloacetate decarboxylase-deficient mutant of Lactococcus lactis IL1403.Journal of bacteriology · 2011Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carbohydrate/citrate cometabolism in Lactococcus lactis results in the formation of the flavor compound acetoin. Resting cells of strain IL1403(pFL3) rapidly consumed citrate while producing acetoin when substoichiometric concentrations of glucose or l-lactate were present. A proton motive force was generated by electrogenic exchange of citrate and lactate catalyzed by the citrate transporter CitP and proton consumption in decarboxylation reactions in the pathway. In the absence of glucose or l-lactate, citrate consumption was biphasic. During the first phase, hardly any citrate was consumed. In the second phase, citrate was converted rapidly, but without the formation of acetoin. Instead, significant amounts of the intermediates pyruvate and α-acetolactate, and the end product acetate, were excreted from the cells. It is shown that the intermediates and acetate are excreted in exchange with the uptake of citrate catalyzed by CitP. The availability of exchangeable substrates in the cytoplasm determines both the rate of citrate consumption and the end product profile. It follows that citrate metabolism in L. lactis IL1403(pFL3) splits up in two routes after the formation of pyruvate, one the well-characterized route yielding acetoin and the other a new route yielding acetate. The flux distribution between the two branches changes from 85:15 in the presence of l-lactate to 30:70 in the presence of pyruvate. The proton motive force generated was greatest in the presence of l-lactate and zero in the presence of pyruvate, suggesting that the pathway to acetate does not generate proton motive force.
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