Evidence map›Paper›PMID 21114271›Full record

ArticleAnalytical chemistry2011

Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3' → 5' exonuclease activity.

Chung-Hang Leung, Daniel Shiu-Hin Chan, Bradley Yat-Wah Man, Chuan-Jen Wang, Wing Lam, Yung-Chi Cheng, Wang-Fun Fong, Wen-Luan Wendy Hsiao, Dik-Lung Ma

Open access · bronzeAbstract readLetter
In one paragraph

Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 105 citations in OpenAlex.

  1. Article
  2. Article
  3. Fluorescent Probes of DNA Repair.ACS chemical biology · 2018
    Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Chung-Hang Leung
Daniel Shiu-Hin Chan
Bradley Yat-Wah Man
Chuan-Jen Wang
Wing Lam
Yung-Chi Cheng
Wang-Fun Fong
Wen-Luan Wendy Hsiao
Dik-Lung Ma
Hong Kong Baptist University · HKYale University · US

Funding

BIOCHEMICAL PHARMACOLOGY OF ANTIHIV COMPOUNDSR01AI038204 · NIAID · YALE UNIVERSITY · PI CHENG, YUNG-CHI · 1995 to 2014
$5.2M
NUCLEOSIDE ANALOGS AS ANTICANCER COMPOUNDSR01CA063477 · NCI · YALE UNIVERSITY · PI CHENG, YUNG-CHI · 1996 to 2007
$2.4M
Nucleoside Analogs as Anticancer CompoundsU01CA063477 · NCI · YALE UNIVERSITY · PI CHENG, YUNG-CHI · 2008 to 2010
$1.9M
NCI NIH HHS CA-063477NCI NIH HHS R01 CA063477NCI NIH HHS U01 CA063477NIAID NIH HHS AI038204NIAID NIH HHS R01 AI038204NIAID NIH HHS R56 AI038204
6 · The paper itself

Abstract

A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3' → 5' exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.

Indexed as

G-QuadruplexesCircular DichroismExodeoxyribonucleasesExonucleasesFluorescent DyesGentian VioletHumansOligonucleotidesPhosphoproteinsSpectrometry, Fluorescenceexodeoxyribonuclease IIIExodeoxyribonucleasesExonucleasesFluorescent DyesGentian VioletOligonucleotidesPhosphoproteinsthree prime repair exonuclease 1

Identifiers

PMID21114271
PMCPMC3021625
OpenAlexW2077341013

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.