ArticleACS chemical neuroscience2010
Automated targeting of cells to electrochemical electrodes using a surface chemistry approach for the measurement of quantal exocytosis.
Article in ACS chemical neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Quantifying neurotransmitter secretion at single-vesicle resolution using high-density complementary metal-oxide-semiconductor electrode array.Nature communications · 2021Article
- Instrumented Microphysiological Systems for Real-Time Measurement and Manipulation of Cellular Electrochemical Processes.iScience · 2019Review
- Electrochemical measurement of quantal exocytosis using microchips.Pflugers Archiv : European journal of physiology · 2018Review
- Two approaches for addressing electrochemical electrode arrays with reduced external connections.Analytical methods : advancing methods and applications · 2015Article
- Carbon fiber ultramicrodic electrode electrodeposited with over-oxidized polypyrrole for amperometric detection of vesicular exocytosis from pheochromocytoma cell.Sensors (Basel, Switzerland) · 2015Article
- Heterogeneous distribution of exocytotic microdomains in adrenal chromaffin cells resolved by high-density diamond ultra-microelectrode arrays.The Journal of physiology · 2014Article
- A microfluidic platform for chemical stimulation and real time analysis of catecholamine secretion from neuroendocrine cells.Lab on a chip · 2013Article
- Electroporation followed by electrochemical measurement of quantal transmitter release from single cells using a patterned microelectrode.Lab on a chip · 2013Article
- Microchip-based electrochemical detection for monitoring cellular systems.Analytical and bioanalytical chemistry · 2013Review
- Parallel recording of neurotransmitters release from chromaffin cells using a 10×10 CMOS IC potentiostat array with on-chip working electrodes.Biosensors & bioelectronics · 2013Article
- Quantification of noise sources for amperometric measurement of quantal exocytosis using microelectrodes.The Analyst · 2012Article
- Microwell device for targeting single cells to electrochemical microelectrodes for high-throughput amperometric detection of quantal exocytosis.Analytical chemistry · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Here we describe a method to fabricate a multi-channel high-throughput microchip device for measurement of quantal transmitter release from individual cells. Instead of bringing carbon-fiber electrodes to cells, the device uses a surface chemistry approach to bring cells to an array of electrochemical microelectrodes. The microelectrodes are small and "cytophilic" in order to promote adhesion of a single cell whereas all other areas of the chip are covered with a thin "cytophobic" film to block cell attachement and facilitate movement of cells to electrodes. This cytophobic film also insulates unused areas of the conductive film, thus the alignment of cell docking sites to working electrodes is automatic. Amperometric spikes resulting from single-granule fusion events were recorded on the device and had amplitudes and kinetics similar to those measured using carbon-fiber microelectrodes. Use of this device will increase the pace of basic neuroscience research and may also find applications in drug discovery or validation.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.