ArticleNucleic acids research2011
Defining cell populations with single-cell gene expression profiling: correlations and identification of astrocyte subpopulations.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- NT2-derived astrocyte-neuron co-culture reflects physiological relevance and offers research validity.Cellular & molecular biology letters · 2025Article
- Astrocytes phenomics as new druggable targets in healthy aging and Alzheimer's disease progression.Frontiers in cellular neuroscience · 2024Review
- Single-Cell RNA-Sequencing in Astrocyte Development, Heterogeneity, and Disease.Cellular and molecular neurobiology · 2023Review
- Formulating treatment of major psychiatric disorders: algorithm targets the dominantly affected brain cell-types.Discover mental health · 2023Review
- Reactive astrogliosis in the era of single-cell transcriptomics.Frontiers in cellular neuroscience · 2023Review
- Activated astrocytes attenuate neocortical seizures in rodent models through driving NaNature communications · 2022Article
- Astrocyte reactivity with late-onset cognitive impairment assessed in vivo usingMolecular psychiatry · 2021Article
- Challenges and Opportunities of Targeting Astrocytes to Halt Neurodegenerative Disorders.Cells · 2021Review
- A top-down measure of gene-to-gene coordination for analyzing cell-to-cell variability.Scientific reports · 2021Article
- Diversity of Reactive Astrogliosis in CNS Pathology: Heterogeneity or Plasticity?Frontiers in cellular neuroscience · 2021Review
- Article
- Neurotoxic potential of reactive astrocytes in canine distemper demyelinating leukoencephalitis.Scientific reports · 2019Article
- SCNS: a graphical tool for reconstructing executable regulatory networks from single-cell genomic data.BMC systems biology · 2018Article
- The Biology of Regeneration Failure and Success After Spinal Cord Injury.Physiological reviews · 2018Review
- Cell Cycle and Cell Size Dependent Gene Expression Reveals Distinct Subpopulations at Single-Cell Level.Frontiers in genetics · 2017Article
- The correlation between expression profiles measured in single cells and in traditional bulk samples.Scientific reports · 2016Article
- Cyclic AMP signaling restricts activation and promotes maturation and antioxidant defenses in astrocytes.BMC genomics · 2016Article
- Processing, visualising and reconstructing network models from single-cell data.Immunology and cell biology · 2016Review
- Identification of Distinct Breast Cancer Stem Cell Populations Based on Single-Cell Analyses of Functionally Enriched Stem and Progenitor Pools.Stem cell reports · 2016Article
- The transcription factor Pax6 contributes to the induction of GLT-1 expression in astrocytes through an interaction with a distal enhancer element.Journal of neurochemistry · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-cell gene expression levels show substantial variations among cells in seemingly homogenous populations. Astrocytes perform many control and regulatory functions in the central nervous system. In contrast to neurons, we have limited knowledge about functional diversity of astrocytes and its molecular basis. To study astrocyte heterogeneity and stem/progenitor cell properties of astrocytes, we used single-cell gene expression profiling in primary mouse astrocytes and dissociated mouse neurosphere cells. The transcript number variability for astrocytes showed lognormal features and revealed that cells in primary cultures to a large extent co-express markers of astrocytes and neural stem/progenitor cells. We show how subpopulations of cells can be identified at single-cell level using unsupervised algorithms and that gene correlations can be used to identify differences in activity of important transcriptional pathways. We identified two subpopulations of astrocytes with distinct gene expression profiles. One had an expression profile very similar to that of neurosphere cells, whereas the other showed characteristics of activated astrocytes in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.