ArticleBiophysical journal2010
The role of the cytoskeleton in volume regulation and beading transitions in PC12 neurites.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Adherent cells sustain membrane tension gradients independently of migration.Nature communications · 2025Article
- Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling.Biomolecules · 2023Article
- JIP3 links lysosome transport to regulation of multiple components of the axonal cytoskeleton.Communications biology · 2022Article
- Midbody-Localized Aquaporin Mediates Intercellular Lumen Expansion During Early Cleavage of an Invasive Freshwater Bivalve.Frontiers in cell and developmental biology · 2022Article
- Poroelastic osmoregulation of living cell volume.iScience · 2021Article
- Axonal spheroids in neurodegeneration.Molecular and cellular neurosciences · 2021Review
- The Roles of Microtubules and Membrane Tension in Axonal Beading, Retraction, and Atrophy.Biophysical journal · 2019Article
- Stem Cell Differentiation is Regulated by Extracellular Matrix Mechanics.Physiology (Bethesda, Md.) · 2018Review
- A model for stretch growth of neurons.Journal of biomechanics · 2016Article
- Amyloid beta oligomers induce neuronal elasticity changes in age-dependent manner: a force spectroscopy study on living hippocampal neurons.Scientific reports · 2016Article
- Tracheal epithelium cell volume responses to hyperosmolar, isosmolar and hypoosmolar solutions: relation to epithelium-derived relaxing factor (EpDRF) effects.Frontiers in physiology · 2013Article
- Mathematical Models of Blast-Induced TBI: Current Status, Challenges, and Prospects.Frontiers in neurology · 2013Article
- The role of stretching in slow axonal transport.Biophysical journal · 2011Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present investigations on volume regulation and beading shape transitions in PC12 neurites, conducted using a flow-chamber technique. By disrupting the cell cytoskeleton with specific drugs, we investigate the role of its individual components in the volume regulation response. We find that microtubule disruption increases both swelling rate and maximum volume attained, but does not affect the ability of the neurite to recover its initial volume. In addition, investigation of axonal beading-also known as pearling instability-provides additional clues on the mechanical state of the neurite. We conclude that volume recovery is driven by passive diffusion of osmolites, and propose that the initial swelling phase is mechanically slowed down by microtubules. Our experiments provide a framework to investigate the role of cytoskeletal mechanics in volume homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.