ArticleBiophysical journal2010
Dynamic adhesion of umbilical cord blood endothelial progenitor cells under laminar shear stress.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Strategies for improved endothelial cell adhesion in microphysiological vascular model systems.PloS one · 2025Article
- Enhancing Mesenchymal Stromal Cell Potency: Inflammatory LicensingFrontiers in immunology · 2022Article
- Whole-Transcriptome Sequencing: a Powerful Tool for Vascular Tissue Engineering and Endothelial Mechanobiology.High-throughput · 2018Review
- Biomaterial-driven in situ cardiovascular tissue engineering-a multi-disciplinary perspective.NPJ Regenerative medicine · 2017Review
- Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells.Stem cells international · 2016Article
- Capture of endothelial cells under flow using immobilized vascular endothelial growth factor.Biomaterials · 2015Article
- Normalization of postinfarct biomechanics using a novel tissue-engineered angiogenic construct.Circulation · 2013Article
- In vitro endothelialization of electrospun terpolymer scaffolds: evaluation of scaffold type and cell source.Tissue engineering. Part A · 2013Article
- Maturing EPCs into endothelial cells: may the force be with the EPCs: focus on "Fluid shear stress induces differentiation of circulating phenotype endothelial progenitor cells".American journal of physiology. Cell physiology · 2012Article
- Endothelial progenitor cells and integrins: adhesive needs.Fibrogenesis & tissue repair · 2012Article
- Parallel-plate flow chamber and continuous flow circuit to evaluate endothelial progenitor cells under laminar flow shear stress.Journal of visualized experiments : JoVE · 2012Article
- Actin stabilization by jasplakinolide affects the function of bone marrow-derived late endothelial progenitor cells.PloS one · 2012Article
- Arachidonic acid randomizes endothelial cell motion and regulates adhesion and migration.PloS one · 2011Article
- Human Cord Blood-Endothelial Progenitor Cells Alleviate Intimal Hyperplasia of Arterial Damage in a Rat Stroke Model.Cell transplantationArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Late outgrowth endothelial progenitor cells (EPCs) represent a promising cell source for rapid reendothelialization of damaged vasculature after expansion ex vivo and injection into the bloodstream. We characterized the dynamic adhesion of umbilical-cord-blood-derived EPCs (CB-EPCs) to surfaces coated with fibronectin. CB-EPC solution density affected the number of adherent cells and larger cells preferentially adhered at lower cell densities. The number of adherent cells varied with shear stress, with the maximum number of adherent cells and the shear stress at maximum adhesion depending upon fluid viscosity. CB-EPCs underwent limited rolling, transiently tethering for short distances before firm arrest. Immediately before arrest, the instantaneous velocity decreased independent of shear stress. A dimensional analysis indicated that adhesion was a function of the net force on the cells, the ratio of cell diffusion to sliding speed, and molecular diffusivity. Adhesion was not limited by the settling rate and was highly specific to α(5)β(1) integrin. Total internal reflection fluorescence microscopy showed that CB-EPCs produced multiple contacts of α(5)β(1) with the surface and the contact area grew during the first 20 min of attachment. These results demonstrate that CB-EPC adhesion from blood can occur under physiological levels of shear stress.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.