ArticleMicrobial cell factories2010
Biosynthesis of chiral 3-hydroxyvalerate from single propionate-unrelated carbon sources in metabolically engineered E. coli.
Article in Microbial cell factories, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A phage DUF669 protein mediates post-cleavage DNA repair against host CRISPR immunity.Nature communications · 2026Article
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth ineLife · 2025Article
- Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth inbioRxiv : the preprint server for biology · 2025Article
- Exploring the Potential of Glutathione Reductase Overexpression to Improve Tellurium Nanoparticle Production inInternational journal of molecular sciences · 2025Article
- Biosensor characterization: formal methods from the perspective of proteome fractions.Synthetic biology (Oxford, England) · 2025Article
- Blended nexus molecules promote COScience advances · 2024Article
- Reverse β-oxidation pathways for efficient chemical production.Journal of industrial microbiology & biotechnology · 2022Article
- Beyond Intracellular Accumulation of Polyhydroxyalkanoates: Chiral Hydroxyalkanoic Acids and Polymer Secretion.Frontiers in bioengineering and biotechnology · 2020Review
- Heterologous expression of 2-methylisoborneol / 2 methylenebornane biosynthesis genes in Escherichia coli yields novel C11-terpenes.PloS one · 2018Article
- Cell foundry with high product specificity and catalytic activity for 21-deoxycortisol biotransformation.Microbial cell factories · 2017Article
- Engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using unrelated carbon sources.Scientific reports · 2016Article
- Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae.Scientific reports · 2016Article
- Functional screening and in vitro analysis reveal thioesterases with enhanced substrate specificity profiles that improve short-chain fatty acid production in Escherichia coli.Applied and environmental microbiology · 2014Article
- Synthetic biology strategies for synthesizing polyhydroxyalkanoates from unrelated carbon sources.Chemical engineering science · 2013Article
- Tailored fatty acid synthesis via dynamic control of fatty acid elongation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone.Nature communications · 2013Article
- Controlled biosynthesis of odd-chain fuels and chemicals via engineered modular metabolic pathways.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Engineering microbial chemical factories to produce renewable "biomonomers".Frontiers in microbiology · 2012Article
- Production in Escherichia coli of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with differing monomer compositions from unrelated carbon sources.Applied and environmental microbiology · 2011Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe ability to synthesize chiral building block molecules with high optical purity is of considerable importance to the fine chemical and pharmaceutical industries. Production of one such compound, 3-hydroxyvalerate (3HV), has previously been studied with respect to the in vivo or in vitro enzymatic depolymerization of biologically-derived co-polymers of poly(3-hydroxybutyrate-co-3-hydroxyvalerate). However, production of this biopolymeric precursor typically necessitates the supplementation of a secondary carbon source (e.g., propionate) into the culture medium. In addition, previous approaches for producing 3HV have not focused on its enantiopure synthesis, and thus suffer from increased costs for product purification.
resultsHere, we report the selective biosynthesis of each 3HV stereoisomer from a single, renewable carbon source using synthetic metabolic pathways in recombinant strains of Escherichia coli. The product chirality was controlled by utilizing two reductases of opposing stereoselectivity. Improvement of the biosynthetic pathway activity and host background was carried out to elevate both the 3HV titers and 3HV/3HB ratios. Overall, shake-flask titers as high as 0.31 g/L and 0.50 g/L of (S)-3HV and (R)-3HV, respectively, were achieved in glucose-fed cultures, whereas glycerol-fed cultures yielded up to 0.19 g/L and 0.96 g/L of (S)-3HV and (R)-3HV, respectively.
conclusionsOur work represents the first report of direct microbial production of enantiomerically pure 3HV from a single carbon source. Continued engineering of host strains and pathway enzymes will ultimately lead to more economical production of chiral 3HV.
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