ArticleVirology journal2010
Identification and characterization of duck plague virus glycoprotein C gene and gene product.
Article in Virology journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Evaluation of safety and immunogenicity of duck-plague virus gC/gE double gene deletion.Frontiers in immunology · 2022Article
- Review
- Isolation and characterization of a duck-origin goose astrovirus in China.Emerging microbes & infections · 2020Article
- Duck Enteritis Virus VP16 Antagonizes IFN-Journal of immunology research · 2020Article
- Molecular characterization and antiapoptotic function analysis of the duck plague virus Us5 gene.Scientific reports · 2019Article
- Molecular characterization of duck enteritis virus UL41 protein.Virology journal · 2018Article
- RNA-seq comparative analysis of Peking ducks spleen gene expression 24 h post-infected with duck plague virulent or attenuated virus.Veterinary research · 2017Article
- Duck enteritis virus UL54 is an IE protein primarily located in the nucleus.Virology journal · 2015Article
- The transcription analysis of duck enteritis virus UL49.5 gene using real-time quantitative reverse transcription PCR.Virus genes · 2013Article
- Comparative genomic analysis of duck enteritis virus strains.Journal of virology · 2012Article
- Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene.Virology journal · 2011Article
- Characterization of the duck enteritis virus UL55 protein.Virology journal · 2011Article
- Characterization of duck enteritis virus UL53 gene and glycoprotein K.Virology journal · 2011Article
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundViral envelope proteins have been proposed to play significant roles in the process of viral infection.
resultsIn this study, an envelope protein gene, gC (NCBI GenBank accession no. EU076811), was expressed and characterized from duck plague virus (DPV), a member of the family herpesviridae. The gene encodes a protein of 432 amino acids with a predicted molecular mass of 45 kDa. Sequence comparisons, multiple alignments and phylogenetic analysis showed that DPV gC has several features common to other identified herpesvirus gC, and was genetically close to the gallid herpervirus.Antibodies raised in rabbits against the pET32a-gC recombinant protein expressed in Escherichia coli BL21 (DE3) recognized a 45-KDa DPV-specific protein from infected duck embryo fibroblast (DEF) cells. Transcriptional and expression analysis, using real-time fluorescent quantitative PCR (FQ-PCR) and Western blot detection, revealed that the transcripts encoding DPV gC and the protein itself appeared late during infection of DEF cells. Immunofluorescence localization further demonstrated that the gC protein exhibited substantial cytoplasm fluorescence in DPV-infected DEF cells.
conclusionsIn this work, the DPV gC protein was successfully expressed in a prokaryotic expression system, and we presented the basic properties of the DPV gC product for the first time. These properties of the gC protein provided a prerequisite for further functional analysis of this gene.
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