ArticleProteomics2010
Quantitative proteomic analysis of A549 cells infected with human respiratory syncytial virus subgroup B using SILAC coupled to LC-MS/MS.
Article in Proteomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Biophysical Characterization of Protein-Protein Interactions in Respiratory Syncytial Virus Infections.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Review
- Proteomic Investigation Reveals Eukaryotic Translation Initiation Factor 5A Involvement in Porcine Reproductive and Respiratory Syndrome Virus InfectionFrontiers in veterinary science · 2022Article
- Evolution of proteomics technologies for understanding respiratory syncytial virus pathogenesis.Expert review of proteomics · 2021Article
- Master Regulator Analysis of the SARS-CoV-2/Human Interactome.Journal of clinical medicine · 2020Article
- Article
- Review
- Chaperoning theViruses · 2018Review
- Article
- Novel insights into human respiratory syncytial virus-host factor interactions through integrated proteomics and transcriptomics analysis.Expert review of anti-infective therapy · 2016Article
- iTRAQ-based quantitative subcellular proteomic analysis of Avibirnavirus-infected cells.Electrophoresis · 2015Article
- Suberoylanilide hydroxamic acid treatment reveals crosstalks among proteome, ubiquitylome and acetylome in non-small cell lung cancer A549 cell line.Scientific reports · 2015Article
- Global and quantitative proteomic analysis of dogs infected by avian-like H3N2 canine influenza virus.Frontiers in microbiology · 2015Article
- Proteomic response to 5,6-dimethylxanthenone 4-acetic acid (DMXAA, vadimezan) in human non-small cell lung cancer A549 cells determined by the stable-isotope labeling by amino acids in cell culture (SILAC) approach.Drug design, development and therapy · 2015Article
- Systems biology unravels interferon responses to respiratory virus infections.World journal of biological chemistry · 2014Review
- Zn-responsive proteome profiling and time-dependent expression of proteins regulated by MTF-1 in A549 cells.PloS one · 2014Article
- Find pairs: the module for protein quantification of the PeakQuant software suite.Omics : a journal of integrative biology · 2012Article
- The interactome of the human respiratory syncytial virus NS1 protein highlights multiple effects on host cell biology.Journal of virology · 2012Article
- The human respiratory syncytial virus nonstructural protein 1 regulates type I and type II interferon pathways.Molecular & cellular proteomics : MCP · 2012Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Human respiratory syncytial virus (HRSV) is a leading cause of serious lower respiratory tract infections in infants. The virus has two subgroups A and B, which differ in prevalence and (nucleotide) sequence. The interaction of subgroup A viruses with the host cell is relatively well characterized, whereas for subgroup B viruses it is not. Therefore quantitative proteomics was used to investigate the interaction of subgroup B viruses with A549 cells, a respiratory cell line. Changes in the cellular proteome and potential canonical pathways were determined using SILAC coupled to LC-MS/MS and Ingenuity Pathway Analysis. To reduce sample complexity and investigate potential trafficking both nuclear and cytoplasmic fractions were analyzed. A total of 904 cellular and six viral proteins were identified and quantified, of which 112 cellular proteins showed a twofold or more change in HRSV-infected cells. Data sets were validated using indirect immunofluorescence confocal microscopy on independent samples. Major changes were observed in constituents of mitochondria including components of the electron transport chain complexes and channels, as well as increases in the abundance of the products of interferon-stimulated genes. This is the first quantitative proteomic analysis of cells infected with HRSV-subgroup B.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.