ArticleChinese journal of integrative medicine2010
Proteomic analysis of chronic restraint stress-induced Gan (肝)-stagnancy syndrome in rats.
Article in Chinese journal of integrative medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Profiling GABA(A) Receptor Subunit Expression in the Hippocampus of PMDD Rat Models Based on TCM Theories.Molecular neurobiology · 2023Article
- Proteomic Analysis of Liver Proteins in a Rat Model of Chronic Restraint Stress-Induced Depression.BioMed research international · 2017Article
- Recent Advance in Applications of Proteomics Technologies on Traditional Chinese Medicine Research.Evidence-based complementary and alternative medicine : eCAM · 2015Review
- Proteomics in Traditional Chinese Medicine with an Emphasis on Alzheimer's Disease.Evidence-based complementary and alternative medicine : eCAM · 2015Review
- Genes underlying positive influence of prenatal environmental enrichment and negative influence of prenatal earthquake simulation and corrective influence of Chinese herbal medicine on rat offspring: Irf7 and Ninj2.African journal of traditional, complementary, and alternative medicines : AJTCAM · 2014Article
- Xiao Yao San Improves Depressive-Like Behavior in Rats through Modulation of β-Arrestin 2-Mediated Pathways in Hippocampus.Evidence-based complementary and alternative medicine : eCAM · 2014Article
- Stress and glucocorticoids increase transthyretin expression in rat choroid plexus via mineralocorticoid and glucocorticoid receptors.Journal of molecular neuroscience : MN · 2012Article
- Effects of buyang huanwu decoction on ventricular remodeling and differential protein profile in a rat model of myocardial infarction.Evidence-based complementary and alternative medicine : eCAM · 2012Article
- A systematic analysis of the theory and practice of syndrome differentiation.Chinese journal of integrative medicine · 2011Review
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo analyze the proteomic characteristics of Gan (肝)-stagnancy syndrome (GSS) by seeking the differential protein in blood and tissues of GSS model rats.
methodsGSS model rats were established by chronic restraint stress, keeping rats in restrain chamber for 6 h every day for 21 successive days. Their blood and liver samples were collected at the end of experiment for differential protein detection with methods of isoelectrofocusing and polyacrylamide SDS-PAGE, silver staining, and scanning. The gel images were analyzed with Imagemaster 2D Elite software, and the excavated differential protein spots were identified with matrix assistant laser resolving TOF mass spectrometry, Western blot, ELISA, and RT-PCR, respectively.
resultsA method for isolating the protein in blood serum and tissues by two-dimensional gel electrophoresis was established and optimized. Six serum proteins and three liver proteins that differentially expressed were identified. The down-regulated differential proteins in serum of GSS model rats were serum albumin precursor, beta 1 globin, antibody against muscle acetylcholine receptor, Ig lambda-2 C region, and transthyretin (TTR), and those in liver tissue were aryl sulfotransferase, enoyl-CoA hydratase, and TTR. TTR down-regulation was found in both serum and liver. Preliminary biological information analysis showed that these differential proteins involved in immune, neuroendocrine, nutrition, and substance metabolism.
conclusionProteomic analysis of differential proteins showed that TTR, aryl sulfotransferase, and enoyl-CoA hydratase expressions are downregulated in the GSS model rats, suggesting that the susceptibility of cancer could be enhanced by chronic stress.
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