ArticlePflugers Archiv : European journal of physiology2011
Effects of hyperpolarization-activated cyclic nucleotide-gated (HCN) channel blockers on the proliferation and cell cycle progression of embryonic stem cells.
Article in Pflugers Archiv : European journal of physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Shared Disease Mechanisms in Neurodevelopmental Disorders: A Cellular and Molecular Biology Perspective.Brain sciences · 2025Review
- The Unique Roles of Ion Channels in Pluripotent Stem Cells in Response to Biological Stimuli.Biology · 2024Review
- Hyperpolarization-activated cyclic nucleotide-gated cation channel 3 promotes HCC development in a female-biased manner.Cell reports · 2023Article
- Highly selective and sensitive detection of glutamate by an electrochemical aptasensor.Analytical and bioanalytical chemistry · 2022Article
- Developmental HCN channelopathy results in decreased neural progenitor proliferation and microcephaly in mice.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The impact of hyperpolarization-activated cyclic nucleotide-gated (HCN) and voltage-gated potassium KCNQ/Kv7 channels on primary microglia function.Journal of neuroinflammation · 2020Article
- Optogenetic stimulation inhibits the self-renewal of mouse embryonic stem cells.Cell & bioscience · 2019Article
- ZD7288, a blocker of the HCN channel family, increases doubling time of mouse embryonic stem cells and modulates differentiation outcomes in a context-dependent manner.SpringerPlus · 2016Article
- L-Type Ca(2+) Channels and SK Channels in Mouse Embryonic Stem Cells and Their Contribution to Cell Proliferation.The Journal of membrane biology · 2015Article
- Effect of optogenetic stimulus on the proliferation and cell cycle progression of neural stem cells.The Journal of membrane biology · 2014Article
- Molecular and functional evidence of HCN4 and caveolin-3 interaction during cardiomyocyte differentiation from human embryonic stem cells.Stem cells and development · 2013Article
- Single mechanosensitive and Ca²⁺-sensitive channel currents recorded from mouse and human embryonic stem cells.The Journal of membrane biology · 2013Article
- Novel roles for hERG K(+) channels in cell proliferation and apoptosis.Cell death & disease · 2011Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem cells (ESCs) can uniquely proliferate indefinitely and differentiate into all cell lineages. ESCs may therefore provide an unlimited supply of cells for cell-based therapies. Previous study reported the presence of hyperpolarization-activated inward currents in undifferentiated mouse (m) ESCs, but the functional role of this hyperpolarization-activated current in mESCs is unknown. In this study, the role of this current in maintaining the proliferative capacity and the cell cycle progression of ESCs was investigated. In D3 mESCs, this hyperpolarization-activated inward current can be blocked by HCN channel blocker ZD7288. Application of the HCN channel blockers, cesium (1-10 mM) or ZD7288 (0.1-30 μM), attenuated cell proliferation in a concentration-dependent manner. Both HCN blockers were found to be non-cytotoxic to mESCs as determined by cell viability test. Interestingly, ZD7288 at 10 and 30 μM was found to decrease the proportion of cells in G(0)/G(1) phase and increase the proportion of cells in S phase. This suggests that this hyperpolarization-activated current can affect the cell cycle progression in mESCs. In summary, the present investigation suggests that ESC proliferation and cell cycle progression can be regulated by this hyperpolarization-activated current.
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