ArticleNephron. Physiology2011
Fluid shear stress induces renal epithelial gene expression through polycystin-2-dependent trafficking of extracellular regulated kinase.
Article in Nephron. Physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- Comparative Proteomic Analysis Reveals Altered Ciliary Proteins in Sickle Cell Disease.Journal of proteome research · 2025Article
- Decoupling shear stress and pressure effects in the biomechanics of autosomal dominant polycystic kidney disease using a perfused kidney-on-chip.bioRxiv : the preprint server for biology · 2024Article
- Tubular deficiency of ABCA1 augments cholesterol- and NaAmerican journal of physiology. Renal physiology · 2024Article
- Glomerular Biomechanical Stress and Lipid Mediators during Cellular Changes Leading to Chronic Kidney Disease.Biomedicines · 2022Review
- Enhanced MCP-1 Release in Early Autosomal Dominant Polycystic Kidney Disease.Kidney international reports · 2021Article
- Unilateral Nephrectomy Stimulates ERK and Is Associated With Enhanced Na Transport.Frontiers in physiology · 2021Article
- Role of chemokines, innate and adaptive immunity.Cellular signalling · 2020Review
- Cellular cholesterol modifies flow-mediated gene expression.American journal of physiology. Renal physiology · 2019Article
- Comprehensive transcriptome analysis of fluid shear stress altered gene expression in renal epithelial cells.Journal of cellular physiology · 2018Article
- Hyperfiltration-associated biomechanical forces in glomerular injury and response: Potential role for eicosanoids.Prostaglandins & other lipid mediators · 2017Review
- A modular microfluidic bioreactor with improved throughput for evaluation of polarized renal epithelial cells.Biomicrofluidics · 2016Article
- Osmolar regulation of endothelin-1 production by the inner medullary collecting duct.Life sciences · 2016Article
- Cholesterol affects flow-stimulated cyclooxygenase-2 expression and prostanoid secretion in the cortical collecting duct.American journal of physiology. Renal physiology · 2015Article
- Flow regulation of endothelin-1 production in the inner medullary collecting duct.American journal of physiology. Renal physiology · 2015Article
- TRPV4 activation mediates flow-induced nitric oxide production in the rat thick ascending limb.American journal of physiology. Renal physiology · 2014Article
- Highly permeable silicon membranes for shear free chemotaxis and rapid cell labeling.Lab on a chip · 2014Article
- Effects of biomechanical forces on signaling in the cortical collecting duct (CCD).American journal of physiology. Renal physiology · 2014Article
- A minimally invasive method for retrieving single adherent cells of different types from cultures.Scientific reports · 2014Article
- Biomechanical regulation of cyclooxygenase-2 in the renal collecting duct.American journal of physiology. Renal physiology · 2014Article
- Prostaglandin E(2) mediates proliferation and chloride secretion in ADPKD cystic renal epithelia.American journal of physiology. Renal physiology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe cilium and cilial proteins have emerged as principal mechanosensors of renal epithelial cells responsible for translating mechanical forces into intracellular signals. Polycystin-2 (PC-2), a cilial protein, regulates flow/shear-induced changes in intracellular Ca(2+) ([Ca(2+)](i)) and recently has been implicated in the regulation of mitogen-activated protein (MAP) kinases. We hypothesize that fluid shear stress (FSS) activates PC-2 which regulates MAP kinase and, in turn, induces MAP kinase-dependent gene expression, specifically, monocyte chemoattractant protein-1 (MCP-1).
methodsTo test this, PC-2 expression was constitutively reduced in a murine inner medullary collecting duct (IMCD3) cell line, and the expression of FSS-induced MCP-1 expression and MAP kinase signaling compared between the parental (PC-2-expressing) and PC-2-deficient IMCD3 cells.
resultsFSS induces MAP kinase signaling and downstream MCP-1 mRNA expression in wild-type IMCD3 cells, while inhibitors of MAP kinase prevented the FSS-induced MCP-1 mRNA response. In contradistinction, FSS did not induce MCP-1 mRNA expression in PC-2-deficient cells, but did increase activation of the upstream MAP kinases. Wild-type cells exposed to FSS augmented the nuclear abundance of activated MAP kinase while PC-2-deficient cells did not.
conclusionsPC-2 regulates FSS-induced MAP kinase trafficking into the nucleus of CD cells.
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