ArticleNucleic acids research2011
Sld2 binds to origin single-stranded DNA and stimulates DNA annealing.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Dpb11 facilitates the colocalization of Mec1-Ddc2 with its activators on gapped DNA.Cell reports · 2026Article
- The yeast Hrq1 helicase stimulates Pso2 translesion nuclease activity and thereby promotes DNA interstrand crosslink repair.The Journal of biological chemistry · 2020Article
- An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-α to Mcm2-7.Nucleic acids research · 2017Article
- Yeast Hrq1 shares structural and functional homology with the disease-linked human RecQ4 helicase.Nucleic acids research · 2017Article
- A Positive Amplification Mechanism Involving a Kinase and Replication Initiation Factor Helps Assemble the Replication Fork Helicase.The Journal of biological chemistry · 2017Article
- Review
- Article
- An Mcm10 Mutant Defective in ssDNA Binding Shows Defects in DNA Replication Initiation.Journal of molecular biology · 2016Article
- The NF-YC-RGL2 module integrates GA and ABA signalling to regulate seed germination in Arabidopsis.Nature communications · 2016Article
- Structural and biochemical characterization of an RNA/DNA binding motif in the N-terminal domain of RecQ4 helicases.Scientific reports · 2016Article
- Mcm10 coordinates the timely assembly and activation of the replication fork helicase.Nucleic acids research · 2016Article
- The Replication Initiation Protein Sld3/Treslin Orchestrates the Assembly of the Replication Fork Helicase during S Phase.The Journal of biological chemistry · 2015Article
- Conserved mechanism for coordinating replication fork helicase assembly with phosphorylation of the helicase.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Dpb11 protein helps control assembly of the Cdc45·Mcm2-7·GINS replication fork helicase.The Journal of biological chemistry · 2015Article
- Switch on the engine: how the eukaryotic replicative helicase MCM2-7 becomes activated.Chromosoma · 2015Review
- The Dbf4-Cdc7 kinase promotes Mcm2-7 ring opening to allow for single-stranded DNA extrusion and helicase assembly.The Journal of biological chemistry · 2015Article
- The intrinsically disordered amino-terminal region of human RecQL4: multiple DNA-binding domains confer annealing, strand exchange and G4 DNA binding.Nucleic acids research · 2014Article
- The replication initiation protein Sld2 regulates helicase assembly.The Journal of biological chemistry · 2014Article
- The N-terminus of the human RecQL4 helicase is a homeodomain-like DNA interaction motif.Nucleic acids research · 2012Article
- Enabling association of the GINS protein tetramer with the mini chromosome maintenance (Mcm)2-7 protein complex by phosphorylated Sld2 protein and single-stranded origin DNA.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sld2 is essential for the initiation of DNA replication, but the mechanism underlying its role in replication is not fully understood. The S-phase cyclin dependent kinase (S-CDK) triggers the association of Sld2 with Dpb11, and a phosphomimetic mutation of Sld2, Sld2T84D, functionally mimics the S-CDK phosphorylated state of Sld2. We report that Sld2T84D binds directly to the single-stranded (ss) DNA of two different origins of replication, and S-CDK phosphorylation of Sld2 stimulates the binding of Sld2 to origin ssDNA. Sld2T84D binds to a thymine-rich ssDNA region of the origin ARS1, and substitution of ARS1 thymines with adenines completely disrupts binding of Sld2T84D. Sld2T84D enhances the ability of origin ssDNA to pulldown Dpb11, and Sld2 binding to origin ssDNA may be important to allow Sld2 and Dpb11 to associate with origin DNA. We also report that Sld2T84D anneals ssDNA of an origin sequence. Dpb11 anneals ssDNA to low levels, and the addition of Sld2T84D with Dpb11 results in higher annealing activity than that of either protein alone. Sld2-stimulated annealing may be important for maintaining genome stability during the initiation of DNA replication.
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