ArticleNucleic acids research2011
Tracking F plasmid TraI relaxase processing reactions provides insight into F plasmid transfer.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Antimicrobial resistance within the one health lens: global drivers, mechanisms and public health policy gaps in low resource settings.Frontiers in cellular and infection microbiology · 2026Review
- Cryo-EM Structure of the relaxosome, a complex essential for bacterial mating and the spread of antibiotic resistance genes.Nature communications · 2025Article
- Delineation of a lactococcal conjugation system reveals a restriction-modification evasion system.Microbial biotechnology · 2023Article
- Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer.Nature communications · 2023Article
- Plasmid Transfer by Conjugation in Gram-Negative Bacteria: From the Cellular to the Community Level.Genes · 2020Review
- Protein Dynamics in F-like Bacterial Conjugation.Biomedicines · 2020Review
- Transcriptome Analysis of Zygotic Induction During Conjugative Transfer of Plasmid RP4.Frontiers in microbiology · 2020Article
- DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island.Nucleic acids research · 2019Article
- From conjugation to T4S systems in Gram-negative bacteria: a mechanistic biology perspective.EMBO reports · 2019Review
- Spread and Persistence of Virulence and Antibiotic Resistance Genes: A Ride on the F Plasmid Conjugation Module.EcoSal Plus · 2018Review
- Article
- Article
- Conjugative DNA Transfer Is Enhanced by Plasmid R1 Partitioning Proteins.Frontiers in molecular biosciences · 2016Article
- Structures of TraI in solution.Journal of molecular modeling · 2014Article
- Common requirement for the relaxosome of plasmid R1 in multiple activities of the conjugative type IV secretion system.Journal of bacteriology · 2014Article
- Review
- Catalytic domain of plasmid pAD1 relaxase TraX defines a group of relaxases related to restriction endonucleases.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Breaking and joining single-stranded DNA: the HUH endonuclease superfamily.Nature reviews. Microbiology · 2013Review
- Structure of a translocation signal domain mediating conjugative transfer by type IV secretion systems.Molecular microbiology · 2013Article
- Origin and fate of the 3' ends of single-stranded DNA generated by conjugal transfer of plasmid R1162.Journal of bacteriology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Early in F plasmid conjugative transfer, the F relaxase, TraI, cleaves one plasmid strand at a site within the origin of transfer called nic. The reaction covalently links TraI Tyr16 to the 5'-ssDNA phosphate. Ultimately, TraI reverses the cleavage reaction to circularize the plasmid strand. The joining reaction requires a ssDNA 3'-hydroxyl; a second cleavage reaction at nic, regenerated by extension from the plasmid cleavage site, may generate this hydroxyl. Here we confirm that TraI is transported to the recipient during transfer. We track the secondary cleavage reaction and provide evidence it occurs in the donor and F ssDNA is transferred to the recipient with a free 3'-hydroxyl. Phe substitutions for four Tyr within the TraI active site implicate only Tyr16 in the two cleavage reactions required for transfer. Therefore, two TraI molecules are required for F plasmid transfer. Analysis of TraI translocation on various linear and circular ssDNA substrates supports the assertion that TraI slowly dissociates from the 3'-end of cleaved F plasmid, likely a characteristic essential for plasmid re-circularization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.