ArticleBMC genomics2010
A microbial detection array (MDA) for viral and bacterial detection.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 138 citations in OpenAlex.
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- Development of DNA Microarray for Indication of Viral Community-Acquired Pneumonia Pathogens.Sovremennye tekhnologii v meditsine · 2025Article
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- Viruses in Wastewater-A Concern for Public Health and the Environment.Microorganisms · 2024Review
- Deciphering the role of female reproductive tract microbiome in reproductive health: a review.Frontiers in cellular and infection microbiology · 2024Review
- DNA microarrays to identify etiological agents, as sensors of environmental wellbeing.Frontiers in bioengineering and biotechnology · 2023Article
- Advances in the oral microbiota and rapid detection of oral infectious diseases.Frontiers in microbiology · 2023Review
- Next Generation Sequencing Approaches to Characterize the Respiratory Tract Virome.Microorganisms · 2022Review
- Article
- Probe design for simultaneous, targeted capture of diverse metagenomic targets.Cell reports methods · 2021Article
- Manipulation of the Gut Microbiome Alters Acetaminophen Biodisposition in Mice.Scientific reports · 2020Article
- Article
- Mosquito-Borne Viruses and Insect-Specific Viruses Revealed in Field-Collected Mosquitoes by a Monitoring Tool Adapted from a Microbial Detection Array.Applied and environmental microbiology · 2019Article
- Understanding and overcoming the pitfalls and biases of next-generation sequencing (NGS) methods for use in the routine clinical microbiological diagnostic laboratory.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- Bovine leukemia virus discovered in human blood.BMC infectious diseases · 2019Article
- Article
- Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis.PloS one · 2019Article
- A highly multiplexed broad pathogen detection assay for infectious disease diagnostics.PLoS neglected tropical diseases · 2018Article
- Impact of viral presence in tumor on gene expression in non-small cell lung cancer.BMC cancer · 2018Article
- Fecal Microbiota Transplantation Is Associated With Reduced Morbidity and Mortality in Porcine Circovirus Associated Disease.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIdentifying the bacteria and viruses present in a complex sample is useful in disease diagnostics, product safety, environmental characterization, and research. Array-based methods have proven utility to detect in a single assay at a reasonable cost any microbe from the thousands that have been sequenced.
methodsWe designed a pan-Microbial Detection Array (MDA) to detect all known viruses (including phages), bacteria and plasmids and developed a novel statistical analysis method to identify mixtures of organisms from complex samples hybridized to the array. The array has broader coverage of bacterial and viral targets and is based on more recent sequence data and more probes per target than other microbial detection/discovery arrays in the literature. Family-specific probes were selected for all sequenced viral and bacterial complete genomes, segments, and plasmids. Probes were designed to tolerate some sequence variation to enable detection of divergent species with homology to sequenced organisms, and to have no significant matches to the human genome sequence.
resultsIn blinded testing on spiked samples with single or multiple viruses, the MDA was able to correctly identify species or strains. In clinical fecal, serum, and respiratory samples, the MDA was able to detect and characterize multiple viruses, phage, and bacteria in a sample to the family and species level, as confirmed by PCR.
conclusionsThe MDA can be used to identify the suite of viruses and bacteria present in complex samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.