ArticleEuropean journal of immunology2010
TLR-mediated signaling pathways circumvent the requirement for DAP12 in mast cells for the induction of inflammatory mediator release.
Article in European journal of immunology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Proteasome-driven modulation of immune and oxidative pathways during scorpion envenomation pathogenesis.The journal of venomous animals and toxins including tropical diseases · 2025Article
- The WNT/β-catenin signaling inhibitor XAV939 enhances the elimination of LNCaP and PC-3 prostate cancer cells by prostate cancer patient lymphocytes in vitro.Scientific reports · 2019Article
- Controlling Mast Cell Activation and Homeostasis: Work Influenced by Bill Paul That Continues Today.Frontiers in immunology · 2018Review
- Molecular mechanisms of macrophage Toll-like receptor-Fc receptor synergy.F1000Research · 2018Review
- Exon skipping of FcεRIβ eliminates expression of the high-affinity IgE receptor in mast cells with therapeutic potential for allergy.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Rictor negatively regulates high-affinity receptors for IgE-induced mast cell degranulation.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Prevention of F-actin assembly switches the response to SCF from chemotaxis to degranulation in human mast cells.European journal of immunology · 2013Article
- A truncated splice-variant of the FcεRIβ receptor subunit is critical for microtubule formation and degranulation in mast cells.Immunity · 2013Article
- A novel KIT-deficient mouse mast cell model for the examination of human KIT-mediated activation responses.Journal of immunological methods · 2013Article
- Stem cell factor programs the mast cell activation phenotype.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Novel identified receptors on mast cells.Frontiers in immunology · 2012Article
- TLR signaling in mast cells: common and unique features.Frontiers in immunology · 2012Article
- mTORC1 and mTORC2 differentially regulate homeostasis of neoplastic and non-neoplastic human mast cells.Blood · 2011Article
- Glycogen synthase kinase-3β is a prosurvival signal for the maintenance of human mast cell homeostasis.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
TLR, expressed on the surface of mast cells, respond to a variety of bacterial and viral components to induce and enhance high-affinity IgE receptor-mediated cytokine production. Recent reports have indicated that specific TLR-dependent responses in macrophages and dendritic cells are regulated by the ITAM-containing molecule, DAP12. When phosphorylated, DAP12 recruits Syk, which is a critical molecule for mast cell activation. We therefore examined whether DAP12 similarly regulates TLR-mediated responses in mast cells. DAP12 was confirmed to be expressed in both human and mouse mast cells and, upon phosphorylation, to recruit Syk. However, although TLR agonists induced cytokine production, and synergistically enhanced high-affinity IgE receptor-mediated cytokine production, surprisingly, they failed to increase DAP12 phosphorylation in mouse bone marrow-derived mast cells (BMMC). Furthermore, normal TLR-mediated responses were observed in DAP12(-/-) BMMC. However, DAP12 phosphorylation and subsequent Syk recruitment were observed in BMMC following Con A-induced aggregation of mannose-glycosylated receptors, and these responses, together with Con A-induced degranulation, were substantially reduced in the DAP12(-/-) BMMC. These data demonstrate that TLR have differential requirements for DAP12 for their function in different cell types and that the inability of TLR to influence mast cell degranulation may be linked to their inability to utilize DAP12 to recruit Syk.
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