ArticleIn vitro cellular & developmental biology. Animal2011
Outgrowth of fibroblast cells from goat skin explants in three different culture media and the establishment of cell lines.
Article in In vitro cellular & developmental biology. Animal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Efficient and simple protocol for mechanical isolation of foreskin-derived primary human dermal fibroblasts for replicative senescence studies.BMC research notes · 2025Article
- NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice.Cell communication and signaling : CCS · 2025Article
- Functional analysis and transcriptome profile of meninges and skin fibroblasts from human-aged donors.Cell proliferation · 2024Article
- Establishment of Capra hircus somatic cells and induction of pluripotent stem-like cells.In vitro cellular & developmental biology. Animal · 2024Article
- Cell culture media dependent in vitro dynamics and culture characteristics of adult caprine dermal fibroblast cells.Scientific reports · 2023Article
- Perspectives of pluripotent stem cells in livestock.World journal of stem cells · 2021Review
- Effects of a traditional Chinese medicine formula and its extraction on muscle fiber characteristics in finishing pigs, porcine cell proliferation and isoforms of myosin heavy chain gene expression in myocytes.Asian-Australasian journal of animal sciences · 2017Article
- Bidirectional crosstalk via IL-6, PGE2 and PGD2 between murine myofibroblasts and alternatively activated macrophages enhances anti-inflammatory phenotype in both cells.British journal of pharmacology · 2016Article
- Recovery of fibroblast-like cells from refrigerated goat skin up to 41 d of animal death.In vitro cellular & developmental biology. Animal · 2015Article
- Clinical protection of goats against CpHV-1 induced genital disease with a BoHV-4-based vector expressing CpHV-1 gD.PloS one · 2013Article
- Establishment and characterization of two fetal fibroblast cell lines from the yak.In vitro cellular & developmental biology. Animal · 2012Article
- Culture of primary bovine chondrocytes on a continuously expanding surface inhibits dedifferentiation.Tissue engineering. Part A · 2012Article
- Effect of postmortem time interval on in vitro culture potential of goat skin tissues stored at room temperature.In vitro cellular & developmental biology. Animal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three different commercially available media, known to support human and porcine-specific fibroblast cultures, were tested for their growth potential on goat skin explants. Although outgrowth of fibroblasts was observed in all media tested, irrespective of breed, porcine-specific media exhibited higher rate of growth. Using this media, three fibroblast cell lines (GSF289, GSF737, and GSF2010) from ear skin explants of normal healthy dairy goats of Kiko and Saanen breed were successfully established in culture. Liquid nitrogen stocks of these frozen cells had a viability rate of 96.2% in in vitro cultures. These cells were morphologically indistinguishable from the cell stocks prior to freezing. Analysis of the growth of a fifth passage culture revealed an 'S' shaped growth curve with a population doubling time of 25 h. The cell lines were found negative for microbial, fungal, and mycoplasma contaminations. These goat skin fibroblast lines and the simple method of their isolation and freezing with high rate of viability will provide additional tools to study molecular mechanisms that regulate fibroblast function and for genetic manipulation of small ruminants.
Indexed as
Identifiers
21108049What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.