ArticleJournal of industrial microbiology & biotechnology2011
A novel docking domain interface model predicting recombination between homoeologous modular biosynthetic gene clusters.
Article in Journal of industrial microbiology & biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 14 citations in OpenAlex.
- Review
- Diverse transcriptional patterns of homoeologous recombinant transcripts in triploid fish (Cyprinidae).Science China. Life sciences · 2021Article
- Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases.Synthetic and systems biotechnology · 2020Review
- Evolution and Diversity of Assembly-Line Polyketide Synthases.Chemical reviews · 2019Review
- Evolution of Efficient Modular Polyketide Synthases by Homologous Recombination.Journal of the American Chemical Society · 2015Article
- Architectures of whole-module and bimodular proteins from the 6-deoxyerythronolide B synthase.Journal of molecular biology · 2014Article
- Evolutionary concepts in natural products discovery: what actinomycetes have taught us.Journal of industrial microbiology & biotechnology · 2014Review
- Databases of the thiotemplate modular systems (CSDB) and their in silico recombinants (r-CSDB).Journal of industrial microbiology & biotechnology · 2013Article
- Annotation of the modular polyketide synthase and nonribosomal peptide synthetase gene clusters in the genome of Streptomyces tsukubaensis NRRL18488.Applied and environmental microbiology · 2012Article
- Horizontal gene transfer and gene conversion drive evolution of modular polyketide synthases.Journal of industrial microbiology & biotechnology · 2012Article
- Combinatorial biosynthesis of polyketides--a perspective.Current opinion in chemical biology · 2012Review
- Recombinatorial biosynthesis of polyketides.Journal of industrial microbiology & biotechnology · 2012Article
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An in silico model for homoeologous recombination between gene clusters encoding modular polyketide synthases (PKS) or non-ribosomal peptide synthetases (NRPS) was developed. This model was used to analyze recombination between 12 PKS clusters from Streptomyces species and related genera to predict if new clusters might give rise to new products. In many cases, there were only a limited number of recombination sites (about 13 per cluster pair), suggesting that recombination may pose constraints on the evolution of PKS clusters. Most recombination events occurred between pairs of ketosynthase (KS) domains, allowing the biosynthetic outcome of the recombinant modules to be predicted. About 30% of recombinants were predicted to produce polyketides. Four NRPS clusters from Streptomyces strains were also used for in silico recombination. They yielded a comparable number of recombinants to PKS clusters, but the adenylation (A) domains contained the largest proportion of recombination events; this might be a mechanism for producing new substrate specificities. The extreme G + C-content, the presence of linear chromosomes and plasmids, as well as the lack of a mutSL-mismatch repair system should favor production of recombinants in Streptomyces species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.