ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2010
GABA depolarizes immature neocortical neurons in the presence of the ketone body ß-hydroxybutyrate.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Trial
- Review
- Article
- The relationship between d-beta-hydroxybutyrate blood concentrations and seizure control in children treated with the ketogenic diet for medically intractable epilepsy.Epilepsia open · 2017Article
- Developmental Changes in Sensory-Evoked Optical Intrinsic Signals in the Rat Barrel Cortex.Frontiers in cellular neuroscience · 2017Article
- An Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Article
- GABA depolarizes immature neurons and inhibits network activity in the neonatal neocortex in vivo.Nature communications · 2015Article
- Commentary: GABA Depolarizes Immature Neurons and Inhibits Network Activity in the Neonatal Neocortex In vivo.Frontiers in pharmacology · 2015Article
- Anion transport and GABA signaling.Frontiers in cellular neuroscience · 2013Review
- Ketone bodies in epilepsy.Journal of neurochemistry · 2012Review
- Synaptic activity-induced Ca(2+) signaling in avian cochlear nucleus magnocellularis neurons.Neuroscience research · 2012Article
- Refuting the challenges of the developmental shift of polarity of GABA actions: GABA more exciting than ever!Frontiers in cellular neuroscience · 2012Article
- Excitatory GABA: How a Correct Observation May Turn Out to be an Experimental Artifact.Frontiers in pharmacology · 2012Article
- Article
- Chronic hyperosmotic stress converts GABAergic inhibition into excitation in vasopressin and oxytocin neurons in the rat.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- The immature brain needs GABA to be excited and hyper-excited.The Journal of physiology · 2011Article
- The metamorphosis of the developing cerebellar microcircuit.Current opinion in neurobiology · 2011Review
- The depolarizing action of GABA in cultured hippocampal neurons is not due to the absence of ketone bodies.PloS one · 2011Article
- Lactate Effectively Covers Energy Demands during Neuronal Network Activity in Neonatal Hippocampal Slices.Frontiers in neuroenergetics · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A large body of evidence suggests that the neurotransmitter GABA undergoes a developmental switch from being predominantly depolarizing-excitatory to predominantly hyperpolarizing-inhibitory. Recently published data, however, point to the possibility that the presumed depolarizing mode of GABA action during early development might represent an artifact due to an insufficient energy supply of the in vitro preparations used. Specifically, addition of the ketone body dl-β-hydroxybutyrate (βHB) to the extracellular medium was shown to prevent GABA from exerting excitatory effects. Applying a complementary set of minimally invasive optical and electrophysiological techniques in brain slices from neonatal mice, we investigated the effects of βHB on GABA actions in immature cells of the upper cortical plate. Fluorescence imaging revealed that GABA-mediated somatic [Ca(2+)] transients, that required activation of GABA(A) receptors and voltage-gated Ca(2+) channels, remained unaffected by βHB. Cell-attached current-clamp recordings showed that, in the presence of βHB, GABA still induced a membrane potential depolarization. To estimate membrane potential changes quantitatively, we used cell-attached recordings of voltage-gated potassium currents and demonstrated that the GABA-mediated depolarization was independent of supplementation of the extracellular solution with βHB. We conclude that, in vitro, GABA depolarizes immature cells of the upper cortical plate in the presence of the ketone body βHB. Our data thereby support the general concept of an excitatory-to-inhibitory switch of GABA action during early development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.