ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2010
Vesicular sterols are essential for synaptic vesicle cycling.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Effects of membrane cholesterol-targeting chemicals on skeletal muscle contractions evoked by direct and indirect stimulation.Journal of muscle research and cell motility · 2024Article
- The translational potential of cholesterol-based therapies for neurological disease.Nature reviews. Neurology · 2023Review
- Phosphorylation of ADAPTOR PROTEIN-2 μ-adaptin by ADAPTOR-ASSOCIATED KINASE1 regulates the tropic growth of Arabidopsis roots.The Plant cell · 2023Article
- Mutants of theInternational journal of molecular sciences · 2021Article
- Disposition of Proteins and Lipids in Synaptic Membrane Compartments Is Altered in Q175/Q7 Huntington's Disease Mouse Striatum.Frontiers in synaptic neuroscience · 2021Article
- Lovastatin Differentially Regulates α7 and α4 Neuronal Nicotinic Acetylcholine Receptor Levels in Rat Hippocampal Neurons.Molecules (Basel, Switzerland) · 2020Article
- Article
- Cholesterol modulates presynaptic and postsynaptic properties of excitatory synaptic transmission.Scientific reports · 2020Article
- The Cell Biology of Tau Secretion.Frontiers in molecular neuroscience · 2020Review
- Reciprocal modulation between amyloid precursor protein and synaptic membrane cholesterol revealed by live cell imaging.Neurobiology of disease · 2019Article
- Cholesterol and the Safety Factor for Neuromuscular Transmission.International journal of molecular sciences · 2019Review
- Lipid Extraction by α-Synuclein Generates Semi-Transmembrane Defects and Lipoprotein Nanoparticles.ACS omega · 2018Article
- Membrane cholesterol mediates the cellular effects of monolayer graphene substrates.Nature communications · 2018Article
- Membrane curvature induced by proximity of anionic phospholipids can initiate endocytosis.Nature communications · 2017Article
- Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.Cell death & disease · 2017Article
- Monitoring activity-dependent bulk endocytosis with the genetically-encoded reporter VAMP4-pHluorin.Journal of neuroscience methods · 2016Article
- Cholesterol-loaded nanoparticles ameliorate synaptic and cognitive function in Huntington's disease mice.EMBO molecular medicine · 2015Article
- Cholesterol regulates multiple forms of vesicle endocytosis at a mammalian central synapse.Journal of neurochemistry · 2015Article
- Differential roles of the mevalonate pathway in the development and survival of mouse Purkinje cells in culture.Molecular neurobiology · 2015Article
- Role of membrane cholesterol in spontaneous exocytosis at frog neuromuscular synapses: reactive oxygen species-calcium interplay.The Journal of physiology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Synaptic vesicles have a high sterol content, but the importance of vesicular sterols during vesicle recycling is unclear. We used the Drosophila temperature-sensitive dynamin mutant, shibire-ts1, to block endocytosis of recycling synaptic vesicles and to trap them reversibly at the plasma membrane where they were accessible to sterol extraction. Depletion of sterols from trapped vesicles prevented recovery of synaptic transmission after removal of the endocytic block. Measurement of vesicle recycling with synaptopHluorin, FM1-43, and FM4-64 demonstrated impaired membrane retrieval after vesicular sterol depletion. When plasma membrane sterols were extracted before vesicle trapping, no vesicle recycling defects were observed. Ultrastructural analysis indicated accumulation of endosomes and a defect in the formation of synaptic vesicles in synaptic terminals subjected to vesicular sterol depletion. Our results demonstrate the importance of a high vesicular sterol concentration for endocytosis and suggest that vesicular and membrane sterol pools do not readily intermingle during vesicle recycling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.