ArticleNucleic acids research2011
Fluorescence-based duplex-quadruplex competition test to screen for telomerase RNA quadruplex ligands.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Probing juxtaposed G-quadruplex and hairpin motifs using a responsive nucleoside probe: a unique scaffold for chemotherapy.Chemical science · 2023Article
- Targeting RNA G-quadruplex with repurposed drugs blocks SARS-CoV-2 entry.PLoS pathogens · 2023Article
- Iso-FRET: an isothermal competition assay to analyze quadruplex formation in vitro.Nucleic acids research · 2022Article
- SARS-CoV-2 Nsp3 unique domain SUD interacts with guanine quadruplexes and G4-ligands inhibit this interaction.Nucleic acids research · 2021Article
- Article
- Mapping and characterization of G-quadruplexes in the genome of the social amoeba Dictyostelium discoideum.Nucleic acids research · 2019Article
- The effects of DNA supercoiling on G-quadruplex formation.Nucleic acids research · 2017Article
- RNA G-quadruplexes: emerging mechanisms in disease.Nucleic acids research · 2017Article
- G-quadruplexes and helicases.Nucleic acids research · 2016Review
- Conformation-sensitive nucleoside analogues as topology-specific fluorescence turn-on probes for DNA and RNA G-quadruplexes.Nucleic acids research · 2015Article
- Interaction of human telomeric DNA with N-methyl mesoporphyrin IX.Nucleic acids research · 2012Article
- The RNA helicase RHAU (DHX36) unwinds a G4-quadruplex in human telomerase RNA and promotes the formation of the P1 helix template boundary.Nucleic acids research · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA and DNA guanine-rich sequences can adopt unusual structures called Guanine quadruplexes (G4). A quadruplex-prone RNA sequence is present at the 5'-end of the 451-nt-long RNA component of telomerase, hTERC. As this quadruplex may interfere with P1 helix formation, a key structural element for this RNA, we are seeking molecules that would alter this RNA duplex-quadruplex equilibrium. In this work, we present a fluorescence-based test designed to identify G4 ligands specific for the hTERC G-rich motif and that can prevent P1 helix formation. From an initial panel of 169 different molecules, 11 were found to be excellent P1 duplex inhibitors. Interestingly, some of the compounds not only exhibit a strong selectivity for quadruplexes over duplexes, but also demonstrated a preference for G4-RNA over all other quadruplexes. This test may easily be adapted to almost any quadruplex-forming sequence and converted into HTS format.
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