ArticleThe Journal of cell biology1990
Brownian motion of inert tracer macromolecules in polymerized and spontaneously bundled mixtures of actin and filamin.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 43 citations in OpenAlex.
- Interplay between Brownian motion and cross-linking controls bundling dynamics in actin networks.Biophysical journal · 2022Article
- Actin filament length tunes elasticity of flexibly cross-linked actin networks.Biophysical journal · 2010Article
- Transient anomalous subdiffusion: effects of specific and nonspecific probe binding with actin gels.The journal of physical chemistry. B · 2010Article
- The riddle of "life," a biologist's critical view.Die Naturwissenschaften · 2009Review
- Mechanics of living cells measured by laser tracking microrheology.Biophysical journal · 2000Article
- Tracer diffusion through F-actin: effect of filament length and cross-linking.Biophysical journal · 1996Article
- Spontaneous vesicle formation at lipid bilayer membranes.Biophysical journal · 1996Article
- Pig kidney (LLC-PK1) cell membrane fluidity during exposure to gentamicin or tobramycin.Antimicrobial agents and chemotherapy · 1994Article
- In vitro models of tail contraction and cytoplasmic streaming in amoeboid cells.The Journal of cell biology · 1993Article
- Affinity of alpha-actinin for actin determines the structure and mechanical properties of actin filament gels.Biophysical journal · 1993Article
- A 27,000-D core of the Dictyostelium 34,000-D protein retains Ca(2+)-regulated actin cross-linking but lacks bundling activity.The Journal of cell biology · 1993Article
- Studies on the structure of actin gels using time correlation spectroscopy of fluorescent beads.Biophysical journal · 1992Article
- The actin filament severing protein actophorin promotes the formation of rigid bundles of actin filaments crosslinked with alpha-actinin.The Journal of cell biology · 1991Article
- Modulation of contraction by gelation/solation in a reconstituted motile model.The Journal of cell biology · 1991Article
- Viscoelastic properties of vimentin compared with other filamentous biopolymer networks.The Journal of cell biology · 1991Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
By use of light microscopy and fluorescence photobleaching recovery, we have studied (a) structures that form in a system composed of copolymerized rabbit muscle actin and chicken gizzard filamin and (b) the Brownian motion of inert tracer macromolecules in this matrix. We have used as tracers size-fractionated fluorescein-labeled ficoll and submicron polystyrene latex particles. In F-actin solutions, the relative diffusion coefficient of the tracer was a decreasing function of both tracer size and actin concentration. Also, a percolation transition for latex particle mobility was found to follow a form suggested by Ogston (Ogston, A. G. 1958. Trans. Faraday Soc. 54:1754-1757) for random filament matrices. The inclusion of filamin before polymerization resulted in increased tracer mobility. Below a filamin dimer-to-actin monomer ratio of 1:140, no structural features were observed in the light microscope. At or above this ratio for all actin concentrations tested, a three-dimensional network of filament bundles was clearly discriminated. Latex particles were always excluded from the bundles. By use of a dialysis optical cell in which polymerization could be initiated with very little hydrodynamic stress, we found that filamin can spontaneously bundle F-actin. A simple physical picture explains how dynamics can affect the structural result of coassembly and provides a further hypothesis on the balance between random filament cross-linking and large-scale bundling. Control of this balance may be important in cytoplasmic motile events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.