ArticleBiomacromolecules2010
Quantifying osteogenic cell degradation of silk biomaterials.
Article in Biomacromolecules, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 59 citations in OpenAlex.
- Recent Advances in Silk Fibroin Derived fromJournal of functional biomaterials · 2025Review
- Biodegradable Silk Fibroin Matrices for Wound Closure in a Human 3D Ex Vivo Approach.Materials (Basel, Switzerland) · 2024Article
- Concentric-mineralized hybrid silk-based scaffolds for bone tissue engineeringJournal of materials chemistry. B · 2023Article
- A Comprehensive Review on Silk Fibroin as a Persuasive Biomaterial for Bone Tissue Engineering.International journal of molecular sciences · 2023Review
- Silk Film Stiffness Modulates Corneal Epithelial Cell Mechanosignaling.Macromolecular chemistry and physics · 2021Article
- The molecular conformation of silk fibroin regulates osteogenic cell behavior by modulating the stability of the adsorbed protein-material interface.Bone research · 2021Article
- The Materiobiology of Silk: Exploring the Biophysical Influence of Silk Biomaterials on Directing Cellular Behaviors.Frontiers in bioengineering and biotechnology · 2021Review
- Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Enzymatic Degradation ofBiomacromolecules · 2020Review
- Bioresorbable silk grafts for small diameter vascular tissue engineering applications: In vitro and in vivo functional analysis.Acta biomaterialia · 2020Article
- Smart Carriers and Nanohealers: A Nanomedical Insight on Natural Polymers.Materials (Basel, Switzerland) · 2017Review
- Evaluation of Silk Inverse Opals for "Smart" Tissue Culture.ACS omega · 2017Article
- Treatment with solubilized Silk-Derived Protein (SDP) enhances rabbit corneal epithelial wound healing.PloS one · 2017Article
- Elastomers in vascular tissue engineering.Current opinion in biotechnology · 2016Review
- Human mesenchymal stem cells cultured on silk hydrogels with variable stiffness and growth factor differentiate into mature smooth muscle cell phenotype.Acta biomaterialia · 2016Article
- In vivo bioresponses to silk proteins.Biomaterials · 2015Review
- Topographical cues regulate the crosstalk between MSCs and macrophages.Biomaterials · 2015Article
- Quantitative characterization of mineralized silk film remodeling during long-term osteoblast-osteoclast co-culture.Biomaterials · 2014Article
- Cell-tethered ligands modulate bone remodeling by osteoblasts and osteoclasts.Advanced functional materials · 2014Article
- Effects of clodronate and alendronate on osteoclast and osteoblast co-cultures on silk-hydroxyapatite films.Acta biomaterialia · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The degradation of silk protein films by human mesenchymal stem cells (hMSCs), osteoblasts and osteoclasts, cells involved in osteogenic functions in normal and diseased bone, was assessed in vitro. The involvement of specific matrix metalloproteinases (MMPs) and integrin signaling in the degradation process was determined. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used to quantitatively compare degradation by the different cell types using surface patterned silk films. Osteoblasts and osteoclasts demonstrated significant degradation of the silk films in vitro in comparison to the hMSCs and the film controls without cells. The osteoclasts degraded the silk films the most and also generated the highest level of MMPs 1 and 2. The osteoblasts upregulated integrins α5 and β1, while the osteoclasts upregulated integrins α2 and β1. There was significant contrast in responses on the silk matrices between osteogenic cells versus undifferentiated hMSCs to illustrate in vitro the role of cell type on matrix remodeling. These are important issues in matching biomaterial matrix features and studies in vitro to remodeling in vivo, in both normal and disease tissue systems. Cell populations and niche factors impact tissue regeneration, wound healing, physiological state, and the ability to better understand the role of different cell types is critical to overall regenerative outcomes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.