Evidence map›Paper›PMID 21104441›Full record

ArticleMolecular biology reports2011

Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes.

Faqian Xiong, Ruichun Zhong, Zhuqiang Han, Jing Jiang, Liangqiong He, Weijian Zhuang, Ronghua Tang

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Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.

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45citing papers in PubMed, 1 pooled it
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3 · Its place in the literature

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45 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Faqian XiongCash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007 Guangxi, China.
Ruichun Zhong
Zhuqiang Han
Jing Jiang
Liangqiong He
Weijian Zhuang
Ronghua Tang

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cultivated peanut possesses an extremely narrow genetic basis. Polymorphism is considerably difficult to identify with the use of conventional biochemical and molecular tools. For the purpose of obtaining considerable DNA polymorphisms and fingerprinting cultivated peanut genotypes in a convenient manner, start codon targeted polymorphism technique was used to study genetic diversity and relatedness among 20 accessions of four major botanical varieties of peanut. Of 36 primers screened, 18 primers could produce unambiguous and reproducible bands. All 18 primers generated a total of 157 fragments, with a mean of 8.72 ranging from 4 to 17 per primer. Of 157 bands, 60 (38.22%) were polymorphic. One to seven polymorphic bands were amplified per primer, with 3.33 polymorphic bands on average. Polymorphism per primer ranged from 14.29 to 66.67%, with an average of 36.76%. The results revealed that not all accessions of the same variety were grouped together and high genetic similarity was detected among the tested genotypes based on cluster analysis and genetic distance analysis, respectively. Further, accession-specific markers were observed in several accessions. All these results demonstrated the following: (1) start codon targeted polymorphism technique can be utilized to identify DNA polymorphisms and fingerprint cultivars in domesticated peanut, and (2) it possesses considerable potential for studying genetic diversity and relationships among peanut accessions.

Indexed as

Codon, InitiatorGenetic VariationGenotypePolymorphism, GeneticArachisCluster AnalysisCrops, AgriculturalDNA, PlantGenetic MarkersPhylogenySequence Analysis, DNACodon, InitiatorDNA, PlantGenetic Markers

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.