ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2011
PlGF gene knockdown in human retinal pigment epithelial cells.
Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- PlGF silencing combined with PEDF overexpression: Modeling RPE secretion as potential therapy for retinal neovascularization.Molecular biology reports · 2020Article
- Observational
- Placental growth factor promotes epithelial-mesenchymal transition-like changes in ARPE-19 cells under hypoxia.Molecular vision · 2018Article
- Osmotic induction of placental growth factor in retinal pigment epithelial cells in vitro: contribution of NFAT5 activity.Molecular biology reports · 2016Article
- A novel model of retinopathy of prematurity in normobaric hyperoxic conditions.International journal of ophthalmology · 2016Article
- Simultaneous application of bevacizumab and anti-CTGF antibody effectively suppresses proangiogenic and profibrotic factors in human RPE cells.Molecular vision · 2015Article
- Knockdown of the placental growth factor gene inhibits laser induced choroidal neovascularization in a murine model.Journal of ophthalmic & vision research · 2013Article
- Placental growth factor inhibition for choroidal neovascularization.Journal of ophthalmic & vision research · 2013Article
- PlGF: a multitasking cytokine with disease-restricted activity.Cold Spring Harbor perspectives in medicine · 2012Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo evaluate the knockdown of placental growth factor (PlGF) gene expression in human retinal pigment epithelium (RPE) cells and its effect on cell proliferation, apoptosis and angiogenic potential of RPE cells.
methodsHuman RPE cells were isolated by dispase I solution and cultured in DMEM/F12 supplemented with 10% fetal calf serum (FCS). A small interfering RNA (siRNA) corresponding to PlGF mRNA and a scrambled siRNA (scRNA) were introduced into the cells. Cell proliferation and cell death were examined by ELISA. PlGF mRNA and protein were quantified by real-time polymerase chain reaction (PCR) and western blot. The levels of gene expression for human retinal pigment epithelium-specific protein 65 kDa (RPE65), cellular retinaldehyde-binding protein (CRALBP) and tyrosinase were examined by real-time PCR. The angiogenic activity of RPE cell-derived conditioned media was assayed by a tube formation assay using human umbilical vein endothelial cells (HUVECs).
resultsAt a final siRNA concentration of 20 pmol/ml, the transfection efficiency was about 80%. The amount of PlGF transcripts was reduced to 10% after 36 h of incubation, and the amount of PlGF protein in culture supernatant was significantly decreased. Suppression of PlGF gene had no effect on RPE cell proliferation and survival, and there were no notable changes in the transcript levels of RPE65, CRALBP or tyrosinase for the cultures treated by siRNA cognate to PlGF. Vascular tube formation was efficiently reduced in HUVECs.
conclusionsOur findings present PlGF as a key modulator of angiogenic potential in RPE cells of the human retina.
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