ArticlePlant cell reports2011
Isolation of a LEAFY homolog from Populus tomentosa: expression of PtLFY in P. tomentosa floral buds and PtLFY-IR-mediated gene silencing in tobacco (Nicotiana tabacum).
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Genome-Wide Analysis of C/S1-bZIP Subfamilies inInternational journal of molecular sciences · 2024Article
- Study on the interaction preference between CYCD subclass and CDK family members at the poplar genome level.Scientific reports · 2022Article
- Isolation and Functional Characterization of aInternational journal of molecular sciences · 2022Article
- Comprehensive Analyses of FourInternational journal of molecular sciences · 2022Article
- Altered sucrose metabolism and plant growth in transgenic Populus tomentosa with altered sucrose synthase PtSS3.Transgenic research · 2020Article
- Transcriptome analysis of diploid and triploidPeerJ · 2020Article
- A Global View of Transcriptome Dynamics During Male Floral Bud Development in Populus tomentosa.Scientific reports · 2018Article
- Effect of climate change on bud phenology of young aspen plants (Ecology and evolution · 2017Article
- Transgenic analysis reveals LeACS-1 as a positive regulator of ethylene-induced shikonin biosynthesis in Lithospermum erythrorhizon hairy roots.Plant molecular biology · 2016Article
- Creating Completely Both Male and Female Sterile Plants by Specifically Ablating Microspore and Megaspore Mother Cells.Frontiers in plant science · 2016Article
- Overexpression of AtAP1M3 regulates flowering time and floral development in Arabidopsis and effects key flowering-related genes in poplar.Transgenic research · 2015Article
- Characterization of the sequence and expression pattern of LFY homologues from dogwood species (Cornus) with divergent inflorescence architectures.Annals of botany · 2013Article
- Sexual dimorphism floral microRNA profiling and target gene expression in andromonoecious poplar (Populus tomentosa).PloS one · 2013Article
- Isolation and expression analysis of a LEAFY/FLORICAULA homolog and its promoter from London plane (Platanus acerifolia Willd.).Plant cell reports · 2012Article
- Over-expression of a putative poplar glycosyltransferase gene, PtGT1, in tobacco increases lignin content and causes early flowering.Journal of experimental botany · 2012Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the genetic and molecular mechanisms underlying floral development in Populus tomentosa, we isolated PtLFY, a LEAFY homolog, from a P. tomentosa floral bud cDNA library. DNA gel blot analysis showed that PtLFY is present as a single copy in the genomes of both male and female individuals of P. tomentosa. The genomic copy is composed of three exons and two introns. Relative expression levels of PtLFY in tissues of P. tomentosa were estimated by RT-PCR; our results revealed that PtLFY mRNA is highly abundant in roots and both male and female floral buds. A low level of gene expression was detected in stems and vegetative buds, and no PtLFY-specific transcripts were detected in leaves. PtLFY expression patterns were analyzed during the development of both male and female floral buds in P. tomentosa via real-time quantitative RT-PCR. Continuous, stable and high-level expression of PtLFY-specific mRNA was detected in both male and female floral buds from September 13th to February 25th, but the level of PtLFY transcripts detected in male floral buds was considerably higher than in female floral buds. Our results also showed an inverted repeat PtLFY fragment (PtLFY-IR) effectively blocked flowering of transgenic tobacco plants, and that this effect appeared to be due to post-transcriptional silencing of the endogenous tobacco LFY homologs NFL1 and NFL2.
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