Evidence map›Paper›PMID 21104030›Full record

ArticleMolecular biology reports2011

Developmental and photosynthetic regulation of δ-endotoxin reveals that engineered sugarcane conferring resistance to 'dead heart' contains no toxins in cane juice.

Muhammad Sarwar Khan, Safdar Ali, Javed Iqbal

Abstract read
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Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 29 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Muhammad Sarwar KhanNational Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, PO Box 577, Faisalabad, 38000, Pakistan. sarwarkhan_40@hotmail.com
Safdar Ali
Javed Iqbal
National Institute for Biotechnology and Genetic Engineering · PKUniversity of Agriculture Faisalabad · PKUniversity of the Punjab · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The phosphoenolpyruvate (PEP) carboxylase is regulated at the levels of transcription and post-translation in C4 plants in light and abundantly accumulates in leaf mesophyll cells. We report here developmental and photosynthetic regulation of stably accumulated Bacillus thuringiensis δ-endotoxin under the control of PEP-C promoter in transgenic sugarcane. In young leaves of plants, the transprotein is accumulated to 39% of the levels in mature leaves (135 ng mg(-1)), and is induced with the cell development, from base to tip. Nevertheless, these levels are decreased up to 99.98% in non-photosynthetic cells as cane matures, from top to bottom, suggesting the photosynthesis regulation of δ-endotoxin in cane cells. Further, transgenic plants are highly resistant to 'dead heart'. In these studies, Scirpophaga nivela larvae causing 'dead heart' were killed within one hour of release to the transgenic plants. Therefore, this report may be regarded as the first report that provides a better strategy for developing transgenic sugarcane lines with absolute protection against invading larvae and no toxin residues in cane juice.

Indexed as

AnimalsBacillus thuringiensis ToxinsBacterial ProteinsDNA PrimersEndotoxinsEnzyme-Linked Immunosorbent AssayGene Expression ProfilingGene Expression Regulation, PlantGenetic VectorsHemolysin ProteinsImmunity, InnateLarvaMothsPhosphoenolpyruvate CarboxylasePhotosynthesisPlant DiseasesBacillus thuringiensis ToxinsBacterial ProteinsDNA PrimersEndotoxinsHemolysin Proteinsinsecticidal crystal protein, Bacillus ThuringiensisPhosphoenolpyruvate CarboxylasePlant Preparations

Identifiers

PMID21104030
OpenAlexW2003476433

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.