ArticlePlant cell reports2011
Protoplast isolation and transient gene expression in the single-cell C4 species, Bienertia sinuspersici.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Advancing our understanding of photosynthesis: discoveries and insights from aquatic plants.Synthetic and systems biotechnology · 2027Review
- Molecular phylogenetics and evolutionary history ofFrontiers in plant science · 2026Review
- Expression and localization of two β-carbonic anhydrases inFrontiers in plant science · 2024Article
- Chloroplast Envelopes Play a Role in the Formation of Autophagy-Related Structures in Plants.Plants (Basel, Switzerland) · 2023Article
- Development of C4 Biochemistry and Change in Expression of Markers for Photosystems I and II in the Single-Cell C4 Species, Bienertia sinuspersiciPlants (Basel, Switzerland) · 2022Article
- Genome-Wide Identification and Analysis of the Phosphoenolpyruvate Carboxylase Gene Family inFrontiers in plant science · 2021Article
- Optimization of isolation and transfection conditions of maize endosperm protoplasts.Plant methods · 2020Article
- A draft genome assembly of halophyte Suaeda aralocaspica, a plant that performs C4 photosynthesis within individual cells.GigaScience · 2019Article
- Agrobacterium-mediated transient transformation of Bienertia sinuspersici to assay recombinant protein distribution between dimorphic chloroplasts.Plant cell reports · 2019Article
- A Protoplast Transient Expression System to Enable Molecular, Cellular, and Functional Studies inFrontiers in plant science · 2018Article
- Transit peptide elements mediate selective protein targeting to two different types of chloroplasts in the single-cell C4 species Bienertia sinuspersici.Scientific reports · 2017Article
- Efficient isolation ofPlant methods · 2017Article
- Size matters for single-cell C4 photosynthesis in Bienertia.Journal of experimental botany · 2017Article
- Establishment of transient gene expression systems in protoplasts from Liriodendron hybrid mesophyll cells.PloS one · 2017Article
- A highly efficient maize nucellus protoplast system for transient gene expression and studying programmed cell death-related processes.Plant cell reports · 2015Article
- A simple improved-throughput xylem protoplast system for studying wood formation.Nature protocols · 2014Article
- One decade after the discovery of single-cell C4 species in terrestrial plants: what did we learn about the minimal requirements of C4 photosynthesis?Photosynthesis research · 2014Review
- Exploring mechanisms linked to differentiation and function of dimorphic chloroplasts in the single cell C4 species Bienertia sinuspersici.BMC plant biology · 2014Article
- The C-terminus of Bienertia sinuspersici Toc159 contains essential elements for its targeting and anchorage to the chloroplast outer membrane.Frontiers in plant science · 2014Article
- A transit peptide-like sorting signal at the C terminus directs the Bienertia sinuspersici preprotein receptor Toc159 to the chloroplast outer membrane.The Plant cell · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although transient gene expression using reporters such as green fluorescent protein is a versatile tool for examining gene functions and intracellular protein trafficking, the establishment of a highly efficient gene manipulation method remains a challenge in many plant species. A reliable transformation protocol has not yet been established for the three single-cell C(4) species, despite their potential of serving as model systems for their extraordinary C(4) photosynthetic metabolism. We report the first protocol optimized for isolating a large-scale and homogenous population of protoplasts from chlorenchyma cells of the single-cell C(4) species Bienertia sinuspersici. Cytochemical staining confirmed the preservation of the unusual subcellular compartmentation of organelles in chlorenchyma cells after cell wall digestion. Approximately 84% of isolated protoplasts expressed the reporter fluorescent protein following our optimized polyethylene glycol-mediated transfection procedures. Fluorescent fusion protein tagged with various intracellular sorting signals demonstrated potential use of the transient gene expression system in subcellular protein localization and organelle dynamics studies. Further applications of the current protoplast isolation and transfection techniques in understanding the novel single-cell C(4) photosynthetic mechanism are discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.