ArticlePLoS neglected tropical diseases2010
A novel high throughput assay for anthelmintic drug screening and resistance diagnosis by real-time monitoring of parasite motility.
Article in PLoS neglected tropical diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 161 citations in OpenAlex.
- Assessment of anthelmintic efficacy of mebendazole in school children in six countries where soil-transmitted helminths are endemic.PLoS neglected tropical diseases · 2014Trial
- Geroprotective drug discovery with an AI-enabled assay for heat resistance.bioRxiv : the preprint server for biology · 2026Article
- In vitro evaluation of the anthelmintic activity of citrus flavonoids against free-living and parasitic nematodes.Scientific reports · 2025Article
- Automated larval motility assays reveal links between eprinomectin treatment failure and drug resistance in Haemonchus contortus.Veterinary research · 2025Article
- The Anthelmintic Activity of Stonefish (Toxins · 2025Article
- An automated, high-resolution phenotypic assay for adult Brugia malayi and microfilaria.Scientific reports · 2024Article
- Proteomic differences between extracellular vesicles and extracellular vesicle-depleted excretory/secretory products of barber's pole worm.Parasites & vectors · 2024Article
- Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes.Scientific reports · 2023Article
- Quantifying metabolic activity of Ascaris suum L3 using resazurin reduction.Parasites & vectors · 2023Article
- Anthelmintic-Like Activity and Ultrastructure Changes Produced by Two Polyphenolic Combinations againstPathogens (Basel, Switzerland) · 2023Article
- Development of a hookworm egg hatching assay to determine the ovicidal effects of anthelminthics.Parasites & vectors · 2023Article
- Optimizing the xWORM assay for monitoring hookworm larvae motility.Frontiers in parasitology · 2023Article
- Recent Advances and Potential Future Applications of MALDI-TOF Mass Spectrometry for Identification of Helminths.Diagnostics (Basel, Switzerland) · 2022Review
- Comparative analysis of the anthelmintic efficacy of European heather extracts on Teladorsagia circumcincta and Trichostrongylus colubriformis egg hatching and larval motility.Parasites & vectors · 2022Article
- Sensitivity of Haemonchus contortus to anthelmintics using different in vitro screening assays: a comparative study.Parasites & vectors · 2022Article
- Image recognition based on deep learning inComputational and structural biotechnology journal · 2022Article
- Anthelmintic Agents from African Medicinal Plants: Review and Prospects.Evidence-based complementary and alternative medicine : eCAM · 2022Review
- Natural-Product-Based Solutions for Tropical Infectious Diseases.Clinical microbiology reviews · 2021Review
- The ATP bioluminescence assay: a new application and optimization for viability testing in the parasitic nematode Haemonchus contortus.Veterinary research · 2021Article
- Practical High-Throughput Method to Screen Compounds for Anthelmintic Activity againstMolecules (Basel, Switzerland) · 2021Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHelminth parasites cause untold morbidity and mortality to billions of people and livestock. Anthelmintic drugs are available but resistance is a problem in livestock parasites, and is a looming threat for human helminths. Testing the efficacy of available anthelmintic drugs and development of new drugs is hindered by the lack of objective high-throughput screening methods. Currently, drug effect is assessed by observing motility or development of parasites using laborious, subjective, low-throughput methods. METHODOLOGY/PRINCIPAL
findingsHere we describe a novel application for a real-time cell monitoring device (xCELLigence) that can simply and objectively assess anthelmintic effects by measuring parasite motility in real time in a fully automated high-throughput fashion. We quantitatively assessed motility and determined real time IC(50) values of different anthelmintic drugs against several developmental stages of major helminth pathogens of humans and livestock, including larval Haemonchus contortus and Strongyloides ratti, and adult hookworms and blood flukes. The assay enabled quantification of the onset of egg hatching in real time, and the impact of drugs on hatch rate, as well as discriminating between the effects of drugs on motility of drug-susceptible and -resistant isolates of H. contortus. CONCLUSIONS/SIGNIFICANCE: Our findings indicate that this technique will be suitable for discovery and development of new anthelmintic drugs as well as for detection of phenotypic resistance to existing drugs for the majority of helminths and other pathogens where motility is a measure of pathogen viability. The method is also amenable to use for other purposes where motility is assessed, such as gene silencing or antibody-mediated killing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.