ArticleCell cycle (Georgetown, Tex.)2010
Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast.
Article in Cell cycle (Georgetown, Tex.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- DNA replication protein Cdc45 directly interacts with PCNA via its PIP box in Leishmania donovani and the Cdc45 PIP box is essential for cell survival.PLoS pathogens · 2020Article
- Cdc7 activates replication checkpoint by phosphorylating the Chk1-binding domain of Claspin in human cells.eLife · 2019Article
- Cdc7 kinase stimulates Aurora B kinase in M-phase.Scientific reports · 2019Article
- Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.Cell reports · 2018Article
- Destabilization of the replication fork protection complex disrupts meiotic chromosome segregation.Molecular biology of the cell · 2017Article
- Mrc1/Claspin: a new role for regulation of origin firing.Current genetics · 2017Review
- A novel p53-Cdc7 link induced by genotoxic stress.Cell cycle (Georgetown, Tex.) · 2017Article
- Checkpoint-Independent Regulation of Origin Firing by Mrc1 through Interaction with Hsk1 Kinase.Molecular and cellular biology · 2017Article
- Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.G3 (Bethesda, Md.) · 2016Article
- The DNA-Binding Domain of S. pombe Mrc1 (Claspin) Acts to Enhance Stalling at Replication Barriers.PloS one · 2015Article
- CDC28 phosphorylates Cac1p and regulates the association of chromatin assembly factor I with chromatin.Cell cycle (Georgetown, Tex.) · 2015Article
- Article
- Proteasome-dependent degradation of replisome components regulates faithful DNA replication.Cell cycle (Georgetown, Tex.) · 2013Review
- Article
- Subcellular proteomics reveals a role for nucleo-cytoplasmic trafficking at the DNA replication origin activation checkpoint.Journal of proteome research · 2013Article
- Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint.The Journal of biological chemistry · 2012Article
- Mechanism of cancer cell death induced by depletion of an essential replication regulator.PloS one · 2012Article
- Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.The Journal of cell biology · 2011Article
- A synthetic human kinase can control cell cycle progression in budding yeast.G3 (Bethesda, Md.) · 2011Article
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8 authors at 2 institutions in 2 countries.
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Abstract
In fission yeast, replication fork arrest activates the replication checkpoint effector kinase Cds1(Chk2/Rad53) through the Rad3(ATR/Mec1)-Mrc1(Claspin) pathway. Hsk1, the Cdc7 homologue of fission yeast required for efficient initiation of DNA replication, is also required for Cds1 activation. Hsk1 kinase activity is required for induction and maintenance of Mrc1 hyperphosphorylation, which is induced by replication fork block and mediated by Rad3. Rad3 kinase activity does not change in an hsk1 temperature-sensitive mutant, and Hsk1 kinase activity is not affected by rad3 mutation. Hsk1 kinase vigorously phosphorylates Mrc1 in vitro, predominantly at non-SQ/TQ sites, but this phosphorylation does not seem to affect the Rad3 action on Mrc1. Interestingly, the replication stress-induced activation of Cds1 and hyperphosphorylation of Mrc1 is almost completely abrogated in an initiation-defective mutant of cdc45, but not in an mcm2 or polε mutant. The results suggest that Hsk1-mediated loading of Cdc45 onto replication origins may play important roles in replication stress-induced checkpoint.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.