ArticleHuman reproduction (Oxford, England)2011
CTGF expression is up-regulated by PROK1 in early pregnancy and influences HTR-8/Svneo cell adhesion and network formation.
Article in Human reproduction (Oxford, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- ITGB3 is reduced in pregnancies with preeclampsia and its influence on biological behavior of trophoblast cells.Molecular medicine (Cambridge, Mass.) · 2024Article
- Trophoblast fusion in fetal growth restriction is inhibited by CTGF in a cell-cycle-dependent manner.Journal of molecular histology · 2024Article
- Novel Insights into Prokineticin 1 Role in Pregnancy-related Diseases.International journal of medical sciences · 2024Review
- Prokineticin 1-prokineticin receptor 1 signaling in trophoblast promotes embryo implantation and placenta development.Scientific reports · 2021Article
- Analysis and identification of novel biomarkers involved in neuroblastoma via integrated bioinformatics.Investigational new drugs · 2021Article
- Cyclin G2 Is Involved in the Proliferation of Placental Trophoblast Cells and Their Interactions with Endothelial Cells.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
- Article
- Endocrine Gland-Derived Vascular Endothelial Growth Factor/Prokineticin-1 in Cancer Development and Tumor Angiogenesis.International journal of endocrinology · 2017Review
- The interplay between glucose and fatty acids on tube formation and fatty acid uptake in the first trimester trophoblast cells, HTR8/SVneo.Molecular and cellular biochemistry · 2015Article
- The role of the CCN family of proteins in female reproduction.Cellular and molecular life sciences : CMLS · 2014Review
- Prokineticin 1 induces a pro-inflammatory response in murine fetal membranes but does not induce preterm delivery.Reproduction (Cambridge, England) · 2013Article
- Pasteurella multocida toxin (PMT) upregulates CTGF which leads to mTORC1 activation in Swiss 3T3 cells.Cellular signalling · 2013Article
- Article
- Prokineticin 1 induces Dickkopf 1 expression and regulates cell proliferation and decidualization in the human endometrium.Molecular human reproduction · 2011Article
- Spatiotemporal expression of SERPINE2 in the human placenta and its role in extravillous trophoblast migration and invasion.Reproductive biology and endocrinology : RB&E · 2011Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
backgroundProkineticin-1 (PROK1) and connective tissue growth factor (CTGF) are expressed in human endometrium and first-trimester decidua and have individually been proposed to have roles in implantation and placentation. We have recently demonstrated that CTGF may be a target gene for PROK1 in gene array analysis of a prokineticin receptor-1 stably transfected Ishikawa endometrial epithelial cell line (PROKR1-Ishikawa). The first aim of the study was to determine the effect of PROK1 on CTGF expression in PROKR1-Ishikawa cells and first-trimester decidua samples. Secondly, the effect of CTGF on trophoblast-derived HTR-8/SVneo cell adhesion and network formation was investigated. METHODS AND
resultsReal-time qPCR showed that CTGF expression is elevated in first-trimester decidua compared with non-pregnant endometrium. In decidua, CTGF co-localized with PROKR1 to the glandular epithelium and a subset of stromal cells. PROK1 increased CTGF mRNA and protein expression in PROKR1-Ishikawa cells and first-trimester human decidua (8-12 weeks gestation). Knock down of endogenous PROK1 using micro RNA constructs targeted at PROK1, resulted in decreased expression of CTGF mRNA and protein in decidua. Inhibitors of specific cell signalling molecules demonstrated that PROK1 regulates CTGF expression via the Gq, phospholipase C (PLC), cSrc, epidermal growth factor receptor (EGFR), mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) kinase pathway activation. Treatment of trophoblast-derived HTR-8/Svneo cells with 1 µg/ml CTGF significantly increased adhesion to collagen IV, and differentiation of the cells into tube-like structures in matrigel.
conclusionsCTGF expression in early pregnancy decidua is regulated by PROK1, via activation of the Gq, PLC, cSrc, EGFR, MAPK/ERK kinase pathway. CTGF in turn may contribute to the regulation of trophoblast conversion of maternal spiral arteries.
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