ArticleDevelopment (Cambridge, England)2010
Initiation of trophectoderm lineage specification in mouse embryos is independent of Cdx2.
Article in Development (Cambridge, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 132 citations in OpenAlex.
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- Nuclear receptor-SINE B1 network modulates expanded pluripotency in blastoids and blastocysts.Nature communications · 2024Article
- Effects of Regulating Hippo and Wnt on the Development and Fate Differentiation of Bovine Embryo.International journal of molecular sciences · 2024Article
- GDF-8 improvesFrontiers in cell and developmental biology · 2024Article
- The number of primitive endoderm cells in the inner cell mass is regulated by platelet-derived growth factor signaling in porcine preimplantation embryos.Animal bioscience · 2023Article
- A Kaleidoscope of Keratin Gene Expression and the Mosaic of Its Regulatory Mechanisms.International journal of molecular sciences · 2023Review
- Early Cell Specification in Mammalian Fertilized and Somatic Cell Nuclear Transfer Embryos.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Journey of the mouse primitive endoderm: from specification to maturation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Article
- Bisphenol Exposure Disrupts Cytoskeletal Organization and Development of Pre-Implantation Embryos.Cells · 2022Article
- Extracellular vesicles improve embryo cryotolerance by maintaining the tight junction integrity during blastocoel re-expansion.Reproduction (Cambridge, England) · 2022Article
- Nested information processing in the living world.Annals of the New York Academy of Sciences · 2021Review
- Roles of cell differentiation factors in preimplantation development of domestic animals.The Journal of reproduction and development · 2021Review
- Lysophosphatidic Acid Accelerates Bovine In Vitro-Produced Blastocyst Formation through the Hippo/YAP Pathway.International journal of molecular sciences · 2021Article
- Reprogramming epiblast stem cells into pre-implantation blastocyst cell-like cells.Stem cell reports · 2021Article
- Chromatin Regulation in Development: Current Understanding and Approaches.Stem cells international · 2021Review
- Cell differentiation events in pre-implantation mouse and bovine embryos.Animal reproduction · 2021Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
The separation of the first two lineages - trophectoderm (TE) and inner cell mass (ICM) - is a crucial event in the development of the early embryo. The ICM, which constitutes the pluripotent founder cell population, develops into the embryo proper, whereas the TE, which comprises the surrounding outer layer, supports the development of the ICM before and after implantation. Cdx2, the first transcription factor expressed specifically in the developing TE, is crucial for the differentiation of cells into the TE, as lack of zygotic Cdx2 expression leads to a failure of embryos to hatch and implant into the uterus. However, speculation exists as to whether maternal Cdx2 is required for initiation of TE lineage separation. Here, we show that effective elimination of both maternal and zygotic Cdx2 transcripts by an RNA interference approach resulted in failure of embryo hatching and implantation, but the developing blastocysts exhibited normal gross morphology, indicating that TE differentiation had been initiated. Expression of keratin 8, a marker for differentiated TE, further confirmed the identity of the TE lineage in Cdx2-deficient embryos. However, these embryos exhibited low mitochondrial activity and abnormal ultrastructure, indicating that Cdx2 plays a key role in the regulation of TE function. Furthermore, we found that embryonic compaction does not act as a 'switch' regulator to turn on Cdx2 expression. Our results clearly demonstrate that neither maternal nor zygotic Cdx2 transcripts direct the initiation of ICM/TE lineage separation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.